Zheng Yadong, Luo Xuenong, Jing Zhizhong, Hu Zhimin, Cai Xuepeng
State Key Laboratory on Veterinary Etiological Biology and Gansu Provincial Key Laboratory of Veterinary Parasitology, Lanzhou Veterinary Research Institute, CAAS, Lanzhou, Gansu 730046, China.
Parasitol Res. 2007 Feb;100(3):645-6. doi: 10.1007/s00436-006-0281-1. Epub 2006 Sep 27.
The partial 18S rRNA sequences of E. coelmaticum and E. pancreaticum were amplified using conserved primers and an evolutionary tree was constructed using Neighbor-Joining. The percent identity of Eurytrema species with other Dicrocoeliidae varied from 97.5 to 98.2, while the percent identity between the two Eurytrema species was up to 99.3. The tree showed that E. coelmaticum and E. pancreaticum were not situated in the same position, and they formed one cluster with L. collurioni. These results support a confirmation with molecular data that E. coelomaticum and E. pancreaticum are different species which apparently were not seriously questioned in the past.
使用保守引物扩增了腔阔盘吸虫和胰阔盘吸虫的部分18S rRNA序列，并采用邻接法构建了进化树。阔盘属物种与其他双腔科物种的序列一致性百分比在97.5%至98.2%之间，而这两种阔盘属物种之间的序列一致性百分比高达99.3%。进化树显示，腔阔盘吸虫和胰阔盘吸虫并不位于同一位置，它们与柯氏阔盘吸虫形成一个聚类。这些结果支持了分子数据的证实，即腔阔盘吸虫和胰阔盘吸虫是不同的物种，这在过去显然没有受到严重质疑。
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