Zheng Ya-dong, Luo Xue-nong, Shi Cheng-hong, Zong Rui-qian, Jing Zhi-zhong, Cai Xue-peng
Key Laboratory of Veterinary Parasitology of Gansu Province, State Key Laboratory of Veterinary Etiological Biology, CAAS, Lanzhou 730046, China.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2006 Oct;24(5):345-8.
To elucidate the taxonomic position of Eurytrema coelmaticum by using molecular technology.
18S rRNA fragment was amplified from E. coelmaticum genomic DNA by specific conservative primers and sequenced. Homology and phylogenic tree of 18S rRNA sequences between E. coelmaticum and other Dicrocoeliidae trematodes were analyzed and constructed by DNAStar and MEGA3 respectively, and their evolutionary relationship was determined.
E. coelmaticum 18S rRNA sequence was with high homology to those from Dicrocoelium dendriticum, Lyperosomum collurionis and Brachylecithum lobatum. Among them, the diversity of E. coelmaticum from D. dendriticum was 2.42%, and that from L. collurionis was 1.75%; D. dendriticum and B. lobatum were closer in evolution only with 1.09% diversity.
For Dicrocoeliidae trematodes, classification based on 18S rRNA target is valid and the sequences are highly conservative. E. coelmaticum is evolutionarily closer to L. collurionis than to D. dendriticum and B. lobatum.
运用分子技术阐明腔阔盘吸虫的分类地位。
采用特异性保守引物从腔阔盘吸虫基因组DNA中扩增出18S rRNA片段并测序。分别用DNAStar和MEGA3软件分析并构建腔阔盘吸虫与其他双腔科吸虫18S rRNA序列的同源性及系统发育树，确定它们的进化关系。
腔阔盘吸虫18S rRNA序列与枝双腔吸虫、结肠副杯叶吸虫和叶状短腺吸虫的18S rRNA序列具有较高同源性。其中，腔阔盘吸虫与枝双腔吸虫的序列差异为2.42%，与结肠副杯叶吸虫的序列差异为1.75%；枝双腔吸虫和叶状短腺吸虫在进化上关系更近，序列差异仅为1.09%。
对于双腔科吸虫，基于18S rRNA靶点的分类是有效的，且序列高度保守。腔阔盘吸虫在进化上与结肠副杯叶吸虫的关系比与枝双腔吸虫和叶状短腺吸虫更近。
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