[湖南省怀化市山羊分离株线粒体和核糖体基因的遗传分析]
[Genetic analysis of mitochondrial and ribosomal genes in isolates from goats in Huaihua City, Hunan Province].
作者信息
Hou Q H, Zhou X H, Yao G M, Li Z B, Shu M, Wang X, Luo W
机构信息
Huaihua Vocational and Technical College, Hunan Province, Huaihua 418000, China.
Xinhuang County Center of Animal Husbandry and Fishery Affairs, Hunan Province, China.
出版信息
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2020 Aug 24;32(4):380-383. doi: 10.16250/j.32.1374.2020126.
OBJECTIVE
To investigate the genetic variation of isolated from goats in Huaihua City, Hunan Province.
METHODS
The partial sequence of mitochondrial cytochrome I () and ribosomal genes were amplified using a PCR assay in isolates from goats in Huaihua City, Hunan Province, and the PCR amplification products were sequenced. Then, the gene sequences were subjected to genetic variation and phylogenetic analyses.
RESULTS
The sequences of the and genes were 430 bp and 1 857 bp in length in 18 isolates from goats in Huaihua City, Hunan Province, and there were 14 and 35 variation sites in and gene sequences, with intra-species genetic variations of 0 to 1.4% and 0 to 0.8%, respectively. The sequences of and genes had 99.0% to 99.8% and 99.5% to 99.8% homologies with those from Chinese strain recorded in the GenBank database. Consistent phylogenetic analysis results were found based on and genes. The 18 isolates from goats in Huaihua City were clustered into a clade with the known isolates registered in GenBank, and the clade with these 18 isolates was close to the clades with species and far from the clades with other trematodes.
CONCLUSIONS
The isolates from goats have a low genetic variation in Huaihua City, Hunan Province. Mitochondrial and ribosomal genes may serve as molecular markers for the studies on the genetic variation in goat-derived .
目的
研究从湖南省怀化市山羊体内分离出的[寄生虫名称未给出]的遗传变异情况。
方法
采用聚合酶链反应（PCR）法扩增来自湖南省怀化市山羊的[寄生虫名称未给出]分离株线粒体细胞色素I（[基因名称未给出]）和核糖体[基因名称未给出]基因的部分序列，并对PCR扩增产物进行测序。然后，对基因序列进行遗传变异和系统发育分析。
结果
来自湖南省怀化市山羊的18株[寄生虫名称未给出]分离株中，[基因名称未给出]和[基因名称未给出]基因序列长度分别为430 bp和1 857 bp，[基因名称未给出]和[基因名称未给出]基因序列分别有14个和35个变异位点，种内遗传变异分别为0至1.4%和0至0.8%。[基因名称未给出]和[基因名称未给出]基因序列与GenBank数据库中记录的中国株的同源性分别为99.0%至99.8%和99.5%至99.8%。基于[基因名称未给出]和[基因名称未给出]基因的系统发育分析结果一致。来自湖南省怀化市山羊的18株[寄生虫名称未给出]分离株与GenBank中登记的已知[寄生虫名称未给出]分离株聚为一个进化枝，包含这18株[寄生虫名称未给出]分离株的进化枝与[寄生虫所属物种名称未给出]物种的进化枝接近，与其他吸虫的进化枝距离较远。
结论
湖南省怀化市山羊的[寄生虫名称未给出]分离株遗传变异较低。线粒体[基因名称未给出]和核糖体[基因名称未给出]基因可作为山羊源[寄生虫名称未给出]遗传变异研究的分子标记。
Zotero 插件