Mohanta Uday Kumar, Ichikawa-Seki Madoka, Hayashi Kei, Itagaki Tadashi
Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, Ueda 3-18-8, Morioka 020-8550, Japan.
Parasitol Res. 2015 Jun;114(6):2099-105. doi: 10.1007/s00436-015-4398-y. Epub 2015 Mar 13.
There is always controversy regarding identification of different species in the genus Eurytrema. Identification has been based mainly on morphology, which can be misleading and subject to differing interpretation among the scientists. Therefore, the aim of this study was to identify Eurytrema flukes both by morphology and molecular properties on the basis of 18-subunit ribosomal RNA (18S rRNA) gene as well as internal transcribed spacer 2 (ITS2) to clarify their phylogenetic status. Among six different agroecological areas of Bangladesh, 22 Eurytrema flukes were recovered from the bile ducts of 22 cattle in Bandarban, a hill district. The flukes were identified as Eurytrema cladorchis through morphometric and morphological studies. Phylogenetic analyses were conducted by neighbor-joining phylogram inferred from both 18S rRNA (1784 bp) gene and ITS2 (229 bp) sequences. A monophyletic clade was constructed by the E. cladorchis from Bangladesh; however, the clade was distinct from those formed by Eurytrema pancreaticum and Eurytrema coelomaticum. This study first described the existence of E. cladorchis from Bangladesh and may provide useful information for both morphological and molecular properties that may further help to clarify phylogenetic relationships within the genus Eurytrema and also for other digeneans.
关于阔盘属不同物种的鉴定一直存在争议。鉴定主要基于形态学，而形态学可能具有误导性，并且科学家之间的解释也存在差异。因此，本研究的目的是基于18亚基核糖体RNA（18S rRNA）基因以及内部转录间隔区2（ITS2），通过形态学和分子特性来鉴定阔盘吸虫，以阐明它们的系统发育地位。在孟加拉国六个不同的农业生态区域中，从山区班达班的22头牛的胆管中采集到了22条阔盘吸虫。通过形态测量和形态学研究，这些吸虫被鉴定为枝睾阔盘吸虫。利用18S rRNA（1784 bp）基因和ITS2（229 bp）序列推断的邻接法系统发育树进行了系统发育分析。来自孟加拉国的枝睾阔盘吸虫构建了一个单系分支；然而，该分支与胰阔盘吸虫和腔阔盘吸虫形成的分支不同。本研究首次描述了孟加拉国枝睾阔盘吸虫的存在，并可能为形态学和分子特性提供有用信息，这可能进一步有助于阐明阔盘属内的系统发育关系，也有助于其他复殖吸虫的研究。
