Hardingham Timothy E, Oldershaw Rachel A, Tew Simon R
UK Centre for Tissue Engineering, Faculty of Life Sciences, University of Manchester, UK.
J Anat. 2006 Oct;209(4):469-80. doi: 10.1111/j.1469-7580.2006.00630.x.
Cartilage repair is an ongoing medical challenge. Tissue engineered solutions to this problem rely on the availability of appropriately differentiated cells in sufficient numbers. This review discusses the potential of primary human articular chondrocytes and mesenchymal stem cells to fulfil this role. Chondrocytes have been transduced with a retrovirus containing the transcription factor SOX9, which permits a greatly improved response of the cells to three-dimensional culture systems, growth factor stimulation and hypoxic culture conditions. Human mesenchymal stem cells have been differentiated into chondrocytes using well-established methods, and the Notch signalling pathway has been studied in detail to establish its role during this process. Both approaches offer insights into these in vitro systems that are invaluable to understanding and designing future cartilage regeneration strategies.
软骨修复是一项持续存在的医学挑战。针对这一问题的组织工程解决方案依赖于足够数量的适当分化细胞的可用性。本综述讨论了原代人关节软骨细胞和间充质干细胞发挥这一作用的潜力。软骨细胞已被含有转录因子SOX9的逆转录病毒转导，这使得细胞对三维培养系统、生长因子刺激和低氧培养条件的反应有了极大改善。人骨髓间充质干细胞已通过成熟方法分化为软骨细胞，并且对Notch信号通路进行了详细研究，以确定其在此过程中的作用。这两种方法都为这些体外系统提供了见解，这对于理解和设计未来的软骨再生策略非常宝贵。
