软骨形成中的软骨、SOX9和Notch信号
Cartilage, SOX9 and Notch signals in chondrogenesis.
作者信息
Hardingham Timothy E, Oldershaw Rachel A, Tew Simon R
机构信息
UK Centre for Tissue Engineering, Faculty of Life Sciences, University of Manchester, UK.
出版信息
J Anat. 2006 Oct;209(4):469-80. doi: 10.1111/j.1469-7580.2006.00630.x.
Abstract
Cartilage repair is an ongoing medical challenge. Tissue engineered solutions to this problem rely on the availability of appropriately differentiated cells in sufficient numbers. This review discusses the potential of primary human articular chondrocytes and mesenchymal stem cells to fulfil this role. Chondrocytes have been transduced with a retrovirus containing the transcription factor SOX9, which permits a greatly improved response of the cells to three-dimensional culture systems, growth factor stimulation and hypoxic culture conditions. Human mesenchymal stem cells have been differentiated into chondrocytes using well-established methods, and the Notch signalling pathway has been studied in detail to establish its role during this process. Both approaches offer insights into these in vitro systems that are invaluable to understanding and designing future cartilage regeneration strategies.
摘要
软骨修复是一项持续存在的医学挑战。针对这一问题的组织工程解决方案依赖于足够数量的适当分化细胞的可用性。本综述讨论了原代人关节软骨细胞和间充质干细胞发挥这一作用的潜力。软骨细胞已被含有转录因子SOX9的逆转录病毒转导，这使得细胞对三维培养系统、生长因子刺激和低氧培养条件的反应有了极大改善。人骨髓间充质干细胞已通过成熟方法分化为软骨细胞，并且对Notch信号通路进行了详细研究，以确定其在此过程中的作用。这两种方法都为这些体外系统提供了见解，这对于理解和设计未来的软骨再生策略非常宝贵。
相似文献
1
Cartilage, SOX9 and Notch signals in chondrogenesis.软骨形成中的软骨、SOX9和Notch信号
J Anat. 2006 Oct;209(4):469-80. doi: 10.1111/j.1469-7580.2006.00630.x.
2
Activation of chondrogenesis in response to injury in normal and transgenic mice with cartilage collagen mutations.正常小鼠和患有软骨胶原蛋白突变的转基因小鼠在损伤后软骨生成的激活情况。
Osteoarthritis Cartilage. 2001;9 Suppl A:S174-9.
3
Pharmacological regulation of adult stem cells: chondrogenesis can be induced using a synthetic inhibitor of the retinoic acid receptor.成体干细胞的药理学调控：软骨生成可通过使用视黄酸受体的合成抑制剂来诱导。
Stem Cells. 2007 Oct;25(10):2460-8. doi: 10.1634/stemcells.2007-0059. Epub 2007 Jul 5.
4
Zinc-finger protein 145, acting as an upstream regulator of SOX9, improves the differentiation potential of human mesenchymal stem cells for cartilage regeneration and repair.锌指蛋白145作为SOX9的上游调节因子，可提高人间充质干细胞向软骨再生和修复方向的分化潜能。
Arthritis Rheum. 2011 Sep;63(9):2711-20. doi: 10.1002/art.30430.
5
The combination of SOX5, SOX6, and SOX9 (the SOX trio) provides signals sufficient for induction of permanent cartilage.SOX5、SOX6和SOX9（SOX三联体）的组合提供了足以诱导永久性软骨形成的信号。
Arthritis Rheum. 2004 Nov;50(11):3561-73. doi: 10.1002/art.20611.
6
[Molecular mechanisms of cartilage formation and chondrocyte maturation].[软骨形成与软骨细胞成熟的分子机制]
Clin Calcium. 2004 Jul;14(7):15-21.
7
Improving chondrogenesis: potential and limitations of SOX9 gene transfer and mechanical stimulation for cartilage tissue engineering.提高软骨形成能力：SOX9 基因转导和机械刺激在软骨组织工程中的潜力和局限性。
Tissue Eng Part A. 2010 Jun;16(6):1845-55. doi: 10.1089/ten.TEA.2009.0531.
8
Contribution of the Interleukin-6/STAT-3 Signaling Pathway to Chondrogenic Differentiation of Human Mesenchymal Stem Cells.白细胞介素-6/STAT3 信号通路对人骨髓间充质干细胞向软骨分化的作用。
Arthritis Rheumatol. 2015 May;67(5):1250-60. doi: 10.1002/art.39036.
9
The role of growth factors in stem cell-directed chondrogenesis: a real hope for damaged cartilage regeneration.生长因子在干细胞定向软骨形成中的作用：受损软骨再生的真正希望。
Int Orthop. 2015 May;39(5):995-1003. doi: 10.1007/s00264-014-2619-0. Epub 2014 Dec 16.
10
The transcription factor Sox9 is involved in BMP-2 signaling.转录因子Sox9参与骨形态发生蛋白-2（BMP-2）信号传导。
J Bone Miner Res. 1999 Oct;14(10):1734-41. doi: 10.1359/jbmr.1999.14.10.1734.
引用本文的文献
1
Enhancement of Chondrogenic Differentiation in Bone Marrow-Derived Stem Cell Spheroids by Methanolic Extract: Insights into Concentration-Dependent mRNA Expression and Gene Clustering Analysis.甲醇提取物对骨髓源性干细胞球团软骨分化的增强作用：浓度依赖性mRNA表达及基因聚类分析的见解
J Pers Med. 2024 Dec 5;14(12):1142. doi: 10.3390/jpm14121142.
2
Local delivery of TGF-β1-mRNA decreases fibrosis in osteochondral defects.局部递送转化生长因子-β1信使核糖核酸可减少骨软骨缺损中的纤维化。
Bioact Mater. 2024 Dec 6;45:509-519. doi: 10.1016/j.bioactmat.2024.11.033. eCollection 2025 Mar.
3
Bergenin protects against osteoarthritis by inhibiting STAT3, NF-κB and Jun pathways and suppressing osteoclastogenesis. Bergenin 通过抑制 STAT3、NF-κB 和 Jun 通路并抑制破骨细胞生成来保护对抗骨关节炎。
Sci Rep. 2024 Aug 31;14(1):20292. doi: 10.1038/s41598-024-71259-9.
4
Reduced Cell Migration in Human Chondrocyte Sheets Increases Tissue Stiffness and Cartilage Protein Production.人软骨细胞片层中细胞迁移减少会增加组织硬度和软骨蛋白的产生。
Tissue Eng Regen Med. 2024 Oct;21(7):1021-1036. doi: 10.1007/s13770-024-00662-0. Epub 2024 Jul 22.
5
Mapping the Single-Cell Differentiation Landscape of Osteosarcoma.绘制骨肉瘤单细胞分化图谱。
Clin Cancer Res. 2024 Aug 1;30(15):3259-3272. doi: 10.1158/1078-0432.CCR-24-0563.
6
Advancements in tissue engineering for articular cartilage regeneration.用于关节软骨再生的组织工程学进展。
Heliyon. 2024 Feb 1;10(3):e25400. doi: 10.1016/j.heliyon.2024.e25400. eCollection 2024 Feb 15.
7
Transcriptomic profiling reveals key early response genes during GDF6-mediated differentiation of human adipose-derived stem cells to nucleus pulposus cells.转录组分析揭示了在生长分化因子6介导的人脂肪来源干细胞向髓核细胞分化过程中的关键早期反应基因。
JOR Spine. 2024 Jan 19;7(1):e1315. doi: 10.1002/jsp2.1315. eCollection 2024 Mar.
8
Emerging structural and pathological analyses on the erectile organ, corpus cavernous containing sinusoids.对包含血窦的勃起器官海绵体进行的新兴结构和病理学分析。
Reprod Med Biol. 2023 Sep 1;22(1):e12539. doi: 10.1002/rmb2.12539. eCollection 2023 Jan-Dec.
9
Genetic models for lineage tracing in musculoskeletal development, injury, and healing.用于肌肉骨骼发育、损伤和修复的谱系追踪的遗传模型。
Bone. 2023 Aug;173:116777. doi: 10.1016/j.bone.2023.116777. Epub 2023 May 6.
10
Adult Stem Cells for Cartilage Regeneration.用于软骨再生的成体干细胞。
Cureus. 2022 Dec 7;14(12):e32280. doi: 10.7759/cureus.32280. eCollection 2022 Dec.
本文引用的文献
1
Update on hip and knee arthroplasty: current state of evidence.髋关节和膝关节置换术的最新进展：证据现状
Arthritis Rheum. 2005 Oct 15;53(5):772-80. doi: 10.1002/art.21465.
2
Roles of bHLH genes in neural stem cell differentiation.bHLH基因在神经干细胞分化中的作用。
Exp Cell Res. 2005 Jun 10;306(2):343-8. doi: 10.1016/j.yexcr.2005.03.015. Epub 2005 Apr 13.
3
The role of type X collagen in facilitating and regulating endochondral ossification of articular cartilage.
Orthod Craniofac Res. 2005 Feb;8(1):11-7. doi: 10.1111/j.1601-6343.2004.00308.x.
4
Retroviral transduction with SOX9 enhances re-expression of the chondrocyte phenotype in passaged osteoarthritic human articular chondrocytes.用SOX9进行逆转录病毒转导可增强传代骨关节炎人关节软骨细胞中软骨细胞表型的重新表达。
Osteoarthritis Cartilage. 2005 Jan;13(1):80-9. doi: 10.1016/j.joca.2004.10.011.
5
Directing stem cell differentiation into the chondrogenic lineage in vitro.体外引导干细胞分化为软骨生成谱系。
Stem Cells. 2004;22(7):1152-67. doi: 10.1634/stemcells.2004-0062.
6
The combination of SOX5, SOX6, and SOX9 (the SOX trio) provides signals sufficient for induction of permanent cartilage.SOX5、SOX6和SOX9（SOX三联体）的组合提供了足以诱导永久性软骨形成的信号。
Arthritis Rheum. 2004 Nov;50(11):3561-73. doi: 10.1002/art.20611.
7
FGF-2 enhances the mitotic and chondrogenic potentials of human adult bone marrow-derived mesenchymal stem cells.成纤维细胞生长因子-2增强人成年骨髓间充质干细胞的有丝分裂和软骨形成潜能。
J Cell Physiol. 2005 May;203(2):398-409. doi: 10.1002/jcp.20238.
8
SOX9-dependent and -independent transcriptional regulation of human cartilage link protein.人软骨连接蛋白的SOX9依赖性和非依赖性转录调控
J Biol Chem. 2004 Dec 3;279(49):50942-8. doi: 10.1074/jbc.M406786200. Epub 2004 Sep 28.
9
Growth factor combination for chondrogenic induction from human mesenchymal stem cell.用于从人骨髓间充质干细胞诱导软骨生成的生长因子组合
Biochem Biophys Res Commun. 2004 Jul 30;320(3):914-9. doi: 10.1016/j.bbrc.2004.06.029.
10
Transduction of passaged human articular chondrocytes with adenoviral, retroviral, and lentiviral vectors and the effects of enhanced expression of SOX9.用腺病毒载体、逆转录病毒载体和慢病毒载体转导传代的人关节软骨细胞以及SOX9表达增强的影响。
Tissue Eng. 2004 Mar-Apr;10(3-4):575-84. doi: 10.1089/107632704323061933.
Zotero 插件