优化的念珠菌生物膜微量滴定法。
Optimized candidal biofilm microtiter assay.
作者信息
Krom Bastiaan P, Cohen Jesse B, McElhaney Feser Gail E, Cihlar Ronald L
机构信息
Department of BioMedical Engineering, University Medical Center Groningen and the University of Groningen, Groningen, The Netherlands.
出版信息
J Microbiol Methods. 2007 Feb;68(2):421-3. doi: 10.1016/j.mimet.2006.08.003. Epub 2006 Sep 26.
Microtiter based candidal biofilm formation is commonly being used. Here we describe the analysis of factors influencing the development of candidal biofilms such as the coating with serum, growth medium and pH. The data reported here show that optimal candidal biofilm formation is obtained when grown in unbuffered YNB at pH 7, in wells that have been coated with Fetal Calf Serum or Fetal Bovine Serum.
基于微量滴定板的念珠菌生物膜形成方法被广泛使用。在此，我们描述了对影响念珠菌生物膜形成的因素的分析，如血清包被、生长培养基和pH值。此处报告的数据表明，当在pH 7的无缓冲酵母氮源基础培养基（YNB）中、在已用胎牛血清包被的孔中生长时，可获得最佳的念珠菌生物膜形成。
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