Dong Yuhe, Yang Chunmiao, Zhong Wenting, Shu Yan, Zhang Yongze, Yang Dongsheng
College of Pharmacy and Food Science, Zhuhai College of Science and Technology, Zhuhai, China.
School of Life Sciences, Jilin University, Changchun, China.
Front Microbiol. 2022 Aug 18;13:974602. doi: 10.3389/fmicb.2022.974602. eCollection 2022.
The inhibitory effects of the anthocyanin obtained from were tested against several food-borne pathogens were evaluated, such as (), (, and In general, anthocyanin had different antibacterial effect on different bacteria, and the best antibacterial effect on , with minimal inhibitory concentration (MIC) of 3.125 mg/mL. Anthocyanin increased the surface hydrophobicity of , discharged the intracellular K, and reduced the total soluble protein, affecting protein synthesis. Fluorescent inverted microscope and flow cytometry (FCM) found a significant increase in fluorescence intensity and lethality relative to the control group, and the dead P3 region to 77.21%. The above suggested a correlation between the antibacterial mechanism of anthocyanin and cell membrane permeability integrity. Biofilm formation was evaluated by the crystal violet assay (CV), silver staining method and methyl thiazolyl tetrazolium (MTT). Scanning electron microscopy (SEM) showed that anthocyanins could change the structure of biofilm.
对从[来源未提及]获得的花青素针对几种食源性病原体的抑制作用进行了测试，例如[具体细菌名称未完整给出]（[相关信息未完整给出]）、[具体细菌名称未完整给出]（[相关信息未完整给出]和[相关信息未完整给出]）。总体而言，花青素对不同细菌具有不同的抗菌作用，对[具体细菌名称未完整给出]的抗菌效果最佳，最低抑菌浓度（MIC）为3.125毫克/毫升。花青素增加了[具体细菌名称未完整给出]的表面疏水性，排出了细胞内的钾，并降低了总可溶性蛋白，影响蛋白质合成。荧光倒置显微镜和流式细胞术（FCM）发现相对于对照组，荧光强度和致死率显著增加，死亡P3区域达到77.21%。上述结果表明花青素的抗菌机制与细胞膜通透性完整性之间存在相关性。通过结晶紫测定法（CV）、银染色法和甲基噻唑基四氮唑（MTT）对生物膜形成进行了评估。扫描电子显微镜（SEM）显示花青素可以改变生物膜的结构。
