Yang Zhifang, Zhou Zhiguang, Huang Gan, Ling He, Yan Xiang, Peng Jian, Li Xia
Institute of Metabolism and Endocrinology, Second Xiangya Hospital, Central South University, Changsha, China.
Diabetes Res Clin Pract. 2007 Apr;76(1):126-31. doi: 10.1016/j.diabres.2006.08.013. Epub 2006 Sep 26.
The latent autoimmune diabetes in adults (LADA) is a subgroup of type 1 diabetes, which procession of autoimmune destruction of beta-cells was slower than classic type 1 diabetes. To investigate the pathogenesis of LADA, we examined the lymphocyte subsets including the CD4(+)CD25(+) T-cells in 60 LADA patients and 30 patients of type 2 diabetes and 30 healthy individuals by FACS. And we compared the expression of FOXP3 mRNA in CD4(+) T-cell between 10 patients of LADA and 10 matched healthy individuals by real time PCR. The percent of CD4(+)CD25(+) T-cells were higher (11.89+/-4.96% versus 8.16+/-3.65%, P<0.01), and the percent CD8(+) T-cells elevated (24.58+/-6.80% versus 19.39+/-7.12, P<0.01) in LADA patients than healthy individuals. While the expression of FOXP3 mRNA in CD4(+) T-cell was markedly decreased in LADA patients (0.52-fold, n=10, P=0.004) compared with normal subjects. In addition, the percent of CD8(+) T-cells related with GAD-Ab titers in LADA patients (r=0.292, P=0.03). Our results showed that there were cellular immune disorder and decreased CD4(+) regulatory T-cells in LADA patients. The adoptive transfer regulatory T-cells seem to be a potential therapeutics for LADA.
成人隐匿性自身免疫性糖尿病（LADA）是1型糖尿病的一个亚组，其β细胞自身免疫性破坏的进程比经典1型糖尿病慢。为了研究LADA的发病机制，我们通过流式细胞术检测了60例LADA患者、30例2型糖尿病患者和30名健康个体的淋巴细胞亚群，包括CD4(+)CD25(+) T细胞。并且我们通过实时PCR比较了10例LADA患者和10名匹配的健康个体CD4(+) T细胞中FOXP3 mRNA的表达。LADA患者中CD4(+)CD25(+) T细胞的百分比更高（11.89±4.96%对8.16±3.65%，P<0.01），且CD8(+) T细胞的百分比升高（24.58±6.80%对19.39±7.12，P<0.01）。与正常受试者相比，LADA患者CD4(+) T细胞中FOXP3 mRNA的表达明显降低（0.52倍，n = 10，P = 0.004）。此外，LADA患者中与GAD - Ab滴度相关的CD8(+) T细胞百分比（r = 0.292，P = 0.03）。我们的结果表明，LADA患者存在细胞免疫紊乱且CD4(+)调节性T细胞减少。过继转移调节性T细胞似乎是LADA的一种潜在治疗方法。
