[成人隐匿性自身免疫性糖尿病患者CD4 + T细胞中FOXP3 mRNA的减少]

[Decrease of FOXP3 mRNA in CD4+ T cells in latent autoimmune diabetes in adult].

作者信息

Yang Zhi-fang, Zhou Zhi-guang, Tang Wei-li, Huang Gan, Peng Jian, Li Xia, He Ling

机构信息

Institute of Metabolism and Endocrinology, Second Xiangya Hospital, Diabetes Center of Central South University, Changsha 410011, China.

出版信息

Zhonghua Yi Xue Za Zhi. 2006 Sep 26;86(36):2533-6.

PMID:17198560

Abstract

OBJECTIVE

To study the percentage of peripheral blood CD4(+)CD25(+) T cells and the expression of FOXP3 mRNA in the patients with latent autoimmune diabetes in adult (LADA).

METHODS

Fresh peripheral blood samples were obtained from 60 patients with LADA, 30 patients with type 2 diabetes and 30 age- and sex-matched matched healthy nondiabetic control subjects without diabetic family history. Two-color staining (anti-CD4, anti-CD25, anti-CD3, and anti-CD8) flow cytometric analysis was employed to measure the CD4(+)CD25(+) T cells. The CD4 positive human cells were isolated with immunomagnetic beads, and then real time-PCR was used to test the expression of FOXP3 mRNA in the CD4(+) T cells.

RESULTS

In the LADA group, the percentage of CD4(+)CD25(+) T cells was 4.1 +/- 1.9, significantly higher than that of the normal control group (2.8 +/- 1.5, P < 0.01), the ratio of CD4(+)CD25(+) to the CD4(+) T cells was 11.9 +/- 5.0, significantly higher than that of the normal control group (8.2 +/- 3.7, P < 0.01), the percentage CD8(+) T cells was 24.6 +/- 6.8, significantly higher than that of the normal control group (19.4 +/- 7.1, P < 0.01) and the CD4(+)/CD8(+) ratio was 1.5 +/- 0.5, significantly lower (1.9 +/- 0.6, P < 0.01). The expression of FOXP3 mRNA in CD4(+) T cells of the LADA group was 0.52 time that of the control group (P < 0.01). The CD4(+)/CD8(+) ratio of LADA group was significantly lower that of the type 2 diabetes group (1.8 +/- 0.8, P < 0.05), however, the other results were not significantly different between these 2 groups. The percentage of was positively correlated with the titer of glutamic acid decarboxylase antibody (GADA) (r = 0.292, P < 0.05).

CONCLUSION

Though the percentage of CD4(+)CD25(+) T cells and the level of CD25 expression in CD4(+) T cells are elevated, the expression of FOXP3 mRNA in CD4(+) T cells is lower. in the patients with LADA. The regulatory T cells may have defective suppressor function in patients with LADA.

摘要

目的

研究成人隐匿性自身免疫性糖尿病（LADA）患者外周血CD4(+)CD25(+) T细胞百分比及FOXP3 mRNA表达情况。

方法

采集60例LADA患者、30例2型糖尿病患者及30例年龄和性别匹配且无糖尿病家族史的健康非糖尿病对照者的新鲜外周血样本。采用双色染色（抗CD4、抗CD25、抗CD3和抗CD8）流式细胞术分析检测CD4(+)CD25(+) T细胞。用免疫磁珠分离CD4阳性人细胞，然后采用实时荧光定量PCR检测CD4(+) T细胞中FOXP3 mRNA的表达。

结果

LADA组CD4(+)CD25(+) T细胞百分比为4.1±1.9，显著高于正常对照组（2.8±1.5，P<0.01）；CD4(+)CD25(+)与CD4(+) T细胞的比值为11.9±5.0，显著高于正常对照组（8.2±3.7，P<0.01）；CD8(+) T细胞百分比为24.6±6.8，显著高于正常对照组（19.4±7.1，P<0.01）；CD4(+)/CD8(+)比值为1.5±0.