Bowick Gavin C, Fennewald Susan M, Elsom Barry L, Aronson Judith F, Luxon Bruce A, Gorenstein David G, Herzog Norbert K
University of Texas Medical Branch, 201 University Blvd., Galveston, TX 77555, USA.
J Virol. 2006 Oct;80(20):10248-52. doi: 10.1128/JVI.01384-06.
The family Arenaviridae includes several National Institutes of Allergy and Infections Diseases category A select agents which cause hemorrhagic fever. There are few vaccines available, and treatment is limited to ribavirin, which varies in efficacy. Development of new antiviral compounds has been hindered by a lack of understanding of the molecular basis of pathogenesis. We used two variants of Pichinde virus, one attenuated and one virulent in the guinea pig model, to delineate the host determinants which lead to either viral clearance or lethal disease. By analyzing protein level changes using pathway analysis, we have identified key intermediates which may be targets for therapeutic intervention.
沙粒病毒科包含几种美国国立过敏与传染病研究所指定的A类生物制剂，可引发出血热。可用的疫苗很少，治疗仅限于利巴韦林，其疗效各异。由于对发病机制的分子基础缺乏了解，新型抗病毒化合物的研发受到了阻碍。我们使用了皮钦德病毒的两种变体，一种在豚鼠模型中减毒，另一种具有毒性，以确定导致病毒清除或致死性疾病的宿主决定因素。通过使用通路分析来分析蛋白质水平的变化，我们确定了可能成为治疗干预靶点的关键中间体。
