Arhel Nathalie, Munier Sandie, Souque Philippe, Mollier Karine, Charneau Pierre
Groupe de Virologie Moléculaire et Vectorologie, Département de Virologie, Institut Pasteur, 25-28 rue du Dr. Roux, 75724 Paris, France.
J Virol. 2006 Oct;80(20):10262-9. doi: 10.1128/JVI.00974-06.
We have previously established, using human immunodeficiency virus type 1 (HIV-1) strain LAI, that the HIV-1 central DNA Flap acts as a cis determinant of viral genome nuclear import. Although the impact of the DNA Flap on nuclear import has already found numerous independent confirmations in the context of lentivirus vectors, it has been claimed that it may be nonessential for infectious virus strains LAI, YU-2 (J. D. Dvorin et al., J. Virol. 76:12087-12096, 2002), HXB2, and NL4-3 (A. Limon et al., J. Virol. 76:12078-12086, 2002). We conducted a detailed analysis of virus infectivity using the provirus clones provided by the authors and analogous target cells. In contrast to published data, our results show that all cPPT mutant viruses exhibit reduced infectivity corresponding to a nuclear import defect irrespective of the viral genetic background or target cell.
我们先前使用1型人类免疫缺陷病毒(HIV-1)LAI毒株证实,HIV-1中央DNA瓣充当病毒基因组核输入的顺式决定因素。尽管在慢病毒载体背景下,DNA瓣对核输入的影响已得到众多独立证实,但有人声称它对于感染性病毒毒株LAI、YU-2(J. D. Dvorin等人,《病毒学杂志》76:12087-12096,2002年)、HXB2和NL4-3(A. Limon等人,《病毒学杂志》76:12078-12086,2002年)可能并非必不可少。我们使用作者提供的原病毒克隆和类似的靶细胞对病毒感染性进行了详细分析。与已发表的数据相反,我们的结果表明,所有cPPT突变病毒均表现出感染性降低,这与核输入缺陷相对应,而与病毒遗传背景或靶细胞无关。