人类免疫缺陷病毒1型DNA瓣突变体的核输入缺陷不依赖于病毒株或靶细胞类型。
Nuclear import defect of human immunodeficiency virus type 1 DNA flap mutants is not dependent on the viral strain or target cell type.
作者信息
Arhel Nathalie, Munier Sandie, Souque Philippe, Mollier Karine, Charneau Pierre
机构信息
Groupe de Virologie Moléculaire et Vectorologie, Département de Virologie, Institut Pasteur, 25-28 rue du Dr. Roux, 75724 Paris, France.
出版信息
J Virol. 2006 Oct;80(20):10262-9. doi: 10.1128/JVI.00974-06.
Abstract
We have previously established, using human immunodeficiency virus type 1 (HIV-1) strain LAI, that the HIV-1 central DNA Flap acts as a cis determinant of viral genome nuclear import. Although the impact of the DNA Flap on nuclear import has already found numerous independent confirmations in the context of lentivirus vectors, it has been claimed that it may be nonessential for infectious virus strains LAI, YU-2 (J. D. Dvorin et al., J. Virol. 76:12087-12096, 2002), HXB2, and NL4-3 (A. Limon et al., J. Virol. 76:12078-12086, 2002). We conducted a detailed analysis of virus infectivity using the provirus clones provided by the authors and analogous target cells. In contrast to published data, our results show that all cPPT mutant viruses exhibit reduced infectivity corresponding to a nuclear import defect irrespective of the viral genetic background or target cell.
摘要
我们先前使用1型人类免疫缺陷病毒（HIV-1）LAI毒株证实，HIV-1中央DNA瓣充当病毒基因组核输入的顺式决定因素。尽管在慢病毒载体背景下，DNA瓣对核输入的影响已得到众多独立证实，但有人声称它对于感染性病毒毒株LAI、YU-2（J. D. Dvorin等人，《病毒学杂志》76:12087-12096，2002年）、HXB2和NL4-3（A. Limon等人，《病毒学杂志》76:12078-12086，2002年）可能并非必不可少。我们使用作者提供的原病毒克隆和类似的靶细胞对病毒感染性进行了详细分析。与已发表的数据相反，我们的结果表明，所有cPPT突变病毒均表现出感染性降低，这与核输入缺陷相对应，而与病毒遗传背景或靶细胞无关。
相似文献
1
Nuclear import defect of human immunodeficiency virus type 1 DNA flap mutants is not dependent on the viral strain or target cell type.人类免疫缺陷病毒1型DNA瓣突变体的核输入缺陷不依赖于病毒株或靶细胞类型。
J Virol. 2006 Oct;80(20):10262-9. doi: 10.1128/JVI.00974-06.
2
HIV-1 genome nuclear import is mediated by a central DNA flap.HIV-1基因组的核输入由一个中央DNA瓣介导。
Cell. 2000 Apr 14;101(2):173-85. doi: 10.1016/S0092-8674(00)80828-4.
3
The central DNA flap of the human immunodeficiency virus type 1 is important for viral replication.人类免疫缺陷病毒1型的中央DNA瓣对病毒复制很重要。
Biochem Biophys Res Commun. 2006 Oct 27;349(3):1100-10. doi: 10.1016/j.bbrc.2006.08.141. Epub 2006 Aug 31.
4
Wild-type and central DNA flap defective HIV-1 lentiviral vector genomes: intracellular visualization at ultrastructural resolution levels.野生型和中心DNA瓣缺陷型HIV-1慢病毒载体基因组：超微结构分辨率水平下的细胞内可视化
Retrovirology. 2006 Jun 26;3:38. doi: 10.1186/1742-4690-3-38.
5
Residual HIV-1 DNA Flap-independent nuclear import of cPPT/CTS double mutant viruses does not support spreading infection.残余 HIV-1 DNA 瓣结构非依赖性核输入的 cPPT/CTS 双突变病毒不能支持病毒的扩散感染。
Retrovirology. 2011 Nov 10;8:92. doi: 10.1186/1742-4690-8-92.
6
Human immunodeficiency virus type 1 KK26-27 matrix mutants display impaired infectivity, circularization and integration but not nuclear import.1型人类免疫缺陷病毒KK26 - 27基质突变体的感染性、环化和整合功能受损，但核输入功能未受影响。
Virology. 2005 Aug 15;339(1):21-30. doi: 10.1016/j.virol.2005.05.023.
7
Positional effects of the central DNA flap in HIV-1-derived lentiviral vectors.HIV-1衍生慢病毒载体中中央DNA瓣的位置效应。
Biochem Biophys Res Commun. 2005 Mar 25;328(4):987-94. doi: 10.1016/j.bbrc.2005.01.052.
8
Evaluation of the functional involvement of human immunodeficiency virus type 1 integrase in nuclear import of viral cDNA during acute infection.评估1型人类免疫缺陷病毒整合酶在急性感染期间病毒cDNA核输入中的功能作用。
J Virol. 2004 Nov;78(21):11563-73. doi: 10.1128/JVI.78.21.11563-11573.2004.
9
Novel nuclear import of Vpr promoted by importin alpha is crucial for human immunodeficiency virus type 1 replication in macrophages.由输入蛋白α促进的Vpr新型核输入对于1型人类免疫缺陷病毒在巨噬细胞中的复制至关重要。
J Virol. 2007 May;81(10):5284-93. doi: 10.1128/JVI.01928-06. Epub 2007 Mar 7.
10
Wild-type levels of nuclear localization and human immunodeficiency virus type 1 replication in the absence of the central DNA flap.在缺乏中央DNA瓣的情况下，核定位和1型人类免疫缺陷病毒复制的野生型水平。
J Virol. 2002 Dec;76(23):12078-86. doi: 10.1128/jvi.76.23.12078-12086.2002.
引用本文的文献
1
Spastin is required for human immunodeficiency virus-1 efficient replication through cooperation with the endosomal sorting complex required for transport (ESCRT) protein.朊病毒蛋白需要与内体分选复合物必需蛋白（ESCRT）蛋白合作，以实现人类免疫缺陷病毒-1 的有效复制。
Virol Sin. 2023 Jun;38(3):448-458. doi: 10.1016/j.virs.2023.05.006. Epub 2023 May 11.
2
CAR-T cell potency: from structural elements to vector backbone components.嵌合抗原受体T细胞效力：从结构元件到载体骨架组件
Biomark Res. 2022 Sep 19;10(1):70. doi: 10.1186/s40364-022-00417-w.
3
RanDeL-Seq: a High-Throughput Method to Map Viral - and -Acting Elements.RanDeL-Seq：一种用于绘制病毒-作用元件的高通量方法。
mBio. 2021 Jan 19;12(1):e01724-20. doi: 10.1128/mBio.01724-20.
4
The Unique, the Known, and the Unknown of Spumaretrovirus Assembly.泡沫反转录病毒组装的独特性、已知性和未知性。
Viruses. 2021 Jan 13;13(1):105. doi: 10.3390/v13010105.
5
Detailed Characterization of Early HIV-1 Replication Dynamics in Primary Human Macrophages.详细描述原发性人巨噬细胞中 HIV-1 复制动力学的早期特征。
Viruses. 2018 Nov 10;10(11):620. doi: 10.3390/v10110620.
6
Phosphorylation of the HIV-1 capsid by MELK triggers uncoating to promote viral cDNA synthesis.MELK对HIV-1衣壳的磷酸化作用引发脱壳，以促进病毒cDNA合成。
PLoS Pathog. 2017 Jul 6;13(7):e1006441. doi: 10.1371/journal.ppat.1006441. eCollection 2017 Jul.
7
Comparative study of the transfection efficiency of commonly used viral vectors in rhesus monkey () brains.恒河猴脑内常用病毒载体转染效率的比较研究。
Zool Res. 2017 Mar 18;38(2):88-95. doi: 10.24272/j.issn.2095-8137.2017.015.
8
Quantitative analysis of the time-course of viral DNA forms during the HIV-1 life cycle.定量分析 HIV-1 生命周期中病毒 DNA 形式的时程。
Retrovirology. 2013 Aug 13;10:87. doi: 10.1186/1742-4690-10-87.
9
The importance of becoming double-stranded: Innate immunity and the kinetic model of HIV-1 central plus strand synthesis.双链的重要性：固有免疫与 HIV-1 中央正链合成的动力学模型。
Virology. 2013 Jun 20;441(1):1-11. doi: 10.1016/j.virol.2013.03.010. Epub 2013 Apr 3.
10
Proteomic analysis of early HIV-1 nucleoprotein complexes.HIV-1 核蛋白复合物的蛋白质组学分析。
J Proteome Res. 2013 Feb 1;12(2):559-72. doi: 10.1021/pr300869h. Epub 2013 Jan 16.
本文引用的文献
1
A single immunization with a minute dose of a lentiviral vector-based vaccine is highly effective at eliciting protective humoral immunity against West Nile virus.用微量基于慢病毒载体的疫苗进行单次免疫，在引发针对西尼罗河病毒的保护性体液免疫方面非常有效。
J Gene Med. 2006 Mar;8(3):265-74. doi: 10.1002/jgm.837.
2
Covert human immunodeficiency virus replication in dendritic cells and in DC-SIGN-expressing cells promotes long-term transmission to lymphocytes.树突状细胞及表达DC-SIGN的细胞中人类免疫缺陷病毒的潜伏复制促进了其向淋巴细胞的长期传播。
J Virol. 2005 May;79(9):5386-99. doi: 10.1128/JVI.79.9.5386-5399.2005.
3
Evidence for gene expression by unintegrated human immunodeficiency virus type 1 DNA species.未整合的1型人类免疫缺陷病毒DNA物种基因表达的证据。
J Virol. 2004 Oct;78(20):11263-71. doi: 10.1128/JVI.78.20.11263-11271.2004.
4
Capsid is a dominant determinant of retrovirus infectivity in nondividing cells.衣壳是逆转录病毒在非分裂细胞中感染性的主要决定因素。
J Virol. 2004 Jun;78(11):5670-8. doi: 10.1128/JVI.78.11.5670-5678.2004.
5
Assessment of the role of the central DNA flap in human immunodeficiency virus type 1 replication by using a single-cycle replication system.利用单周期复制系统评估中央DNA瓣在1型人类免疫缺陷病毒复制中的作用。
J Virol. 2004 Mar;78(6):3170-7. doi: 10.1128/jvi.78.6.3170-3177.2004.
6
Human immunodeficiency virus type 1 DNA nuclear import and integration are mitosis independent in cycling cells.1型人类免疫缺陷病毒DNA的核输入和整合在循环细胞中不依赖有丝分裂。
J Virol. 2003 Dec;77(24):13412-7. doi: 10.1128/jvi.77.24.13412-13417.2003.
7
Lentivirally transduced dendritic cells as a tool for cancer immunotherapy.慢病毒转导的树突状细胞作为癌症免疫治疗的一种工具。
J Gene Med. 2003 Aug;5(8):654-67. doi: 10.1002/jgm.400.
8
A highly efficient, stable, and rapid approach for ex vivo human liver gene therapy via a FLAP lentiviral vector.一种通过FLAP慢病毒载体进行高效、稳定且快速的离体人类肝脏基因治疗方法。
Hepatology. 2003 Jul;38(1):114-22. doi: 10.1053/jhep.2003.50265.
9
Impact of the central polypurine tract on the kinetics of human immunodeficiency virus type 1 vector transduction.中央多聚嘌呤序列对1型人类免疫缺陷病毒载体转导动力学的影响
J Virol. 2003 Apr;77(8):4685-94. doi: 10.1128/jvi.77.8.4685-4694.2003.
10
Reassessment of the roles of integrase and the central DNA flap in human immunodeficiency virus type 1 nuclear import.对整合酶和中央DNA瓣在1型人类免疫缺陷病毒核输入中作用的重新评估。
J Virol. 2002 Dec;76(23):12087-96. doi: 10.1128/jvi.76.23.12087-12096.2002.
Zotero 插件