Wang Won-Bo, Lai Hsin-Chih, Hsueh Po-Ren, Chiou Robin Y-Y, Lin Shwu-Bin, Liaw Shwu-Jen
Department of Laboratory Medicine, National Taiwan University Hospital, Taipei, Taiwan, Republic of China.
Department of Food Science, National Chiayi University, Chiayi, Taiwan, Republic of China.
J Med Microbiol. 2006 Oct;55(Pt 10):1313-1321. doi: 10.1099/jmm.0.46661-0.
Resveratrol (3,5,4-trihydroxy-trans-stilbene) is a phytoalexin compound with anti-inflammatory and antioxidant activities. The effect of resveratrol on swarming and virulence factor expression of Proteus mirabilis, an important pathogen infecting the urinary tract, was determined on swarming agar plates with and without the compound. Bacteria harvested at different times were assayed for cell length and the production of flagella, haemolysin and urease. Resveratrol inhibited P. mirabilis swarming and virulence factor expression in a dose-dependent manner. Resveratrol significantly inhibited swarming at 15 microg ml(-1), and completely inhibited swarming at 60 microg ml(-1). Inhibition of swarming and virulence factor expression was mediated through RsbA, a His-containing phosphotransmitter of the bacterial two-component signalling system possibly involved in quorum sensing. Complementation of an rsbA-defective mutant with the rsbA gene restored its responsiveness to resveratrol. The compound also inhibited the ability of P. mirabilis to invade human urothelial cells. These findings suggest that resveratrol has potential to be developed as an antimicrobial agent against P. mirabilis infection.
白藜芦醇（3,5,4 - 三羟基 - 反式 - 芪）是一种具有抗炎和抗氧化活性的植物抗毒素化合物。在含有和不含该化合物的群游琼脂平板上，测定了白藜芦醇对奇异变形杆菌（一种感染尿路的重要病原体）群游和毒力因子表达的影响。对在不同时间收获的细菌进行细胞长度以及鞭毛、溶血素和尿素酶产生情况的检测。白藜芦醇以剂量依赖的方式抑制奇异变形杆菌的群游和毒力因子表达。白藜芦醇在15微克/毫升时显著抑制群游，在60微克/毫升时完全抑制群游。群游和毒力因子表达的抑制是通过RsbA介导的，RsbA是细菌双组分信号系统中一种含组氨酸的磷酸传递体，可能参与群体感应。用rsbA基因对rsbA缺陷型突变体进行互补恢复了其对白藜芦醇的反应性。该化合物还抑制奇异变形杆菌侵袭人尿道上皮细胞的能力。这些发现表明白藜芦醇有潜力被开发成为一种抗奇异变形杆菌感染的抗菌剂。
