Amante Chiara, De Soricellis Chiara, Luccheo Gianni, Luccheo Luigi, Russo Paola, Aquino Rita Patrizia, Del Gaudio Pasquale
Department of Pharmacy, University of Salerno, Via Giovanni Paolo II, 132, 84084 Fisciano, SA, Italy.
Anvest Health s.r.l., Via Rosario Livatino, 84083 Castel San Giorgio, SA, Italy.
Life (Basel). 2023 Apr 13;13(4):1005. doi: 10.3390/life13041005.
The National Institute of Health has reported that approximately 80% of chronic infections are associated with biofilms, which are indicated as one of the main reasons for bacteria's resistance to antimicrobial agents. Several studies have revealed the role of N-acetylcysteine (NAC), in reducing biofilm formation induced by different microorganisms. A novel mixture made up of NAC and different natural ingredients (bromelain, ascorbic acid, Ribes nigrum, resveratrol, and pelargonium) has been developed in order to obtain a pool of antioxidants as an alternative strategy for biofilm reduction. The study has demonstrated that the mixture is able to significantly enhance NAC activity against different Gram-positive and Gram-negative bacteria. It has shown an increase in NAC permeation in vitro through an artificial fluid, moving from 2.5 to 8 μg/cm after 30 min and from 4.4 to 21.6 μg/cm after 180 min, and exhibiting a strongly fibrinolytic activity compared to the single components of the mixture. Moreover, this novel mixture has exhibited an antibiofilm activity against and the ability to reduce growth by more than 20% in a time-killing assay, while on , and , the growth was reduced by more than 80% compared to NAC. The flogomicina mixture has also been proven capable of reducing bacterial adhesion to abiotic surfaces of , by more than 11% concerning only the NAC. In combination with amoxicillin, it has been shown to significantly increase the drug's effectiveness after 14 days, offering a safe and natural way to reduce the daily dosage of antibiotics in prolonged therapies and consequently, reduce antibiotic resistance.
美国国立卫生研究院报告称，约80%的慢性感染与生物膜有关，生物膜被认为是细菌对抗菌剂产生耐药性的主要原因之一。多项研究揭示了N-乙酰半胱氨酸（NAC）在减少不同微生物诱导的生物膜形成方面的作用。为了获得一组抗氧化剂作为减少生物膜的替代策略，已开发出一种由NAC和不同天然成分（菠萝蛋白酶、抗坏血酸、黑醋栗、白藜芦醇和天竺葵）组成的新型混合物。该研究表明，该混合物能够显著增强NAC对不同革兰氏阳性菌和革兰氏阴性菌的活性。它显示出NAC在体外通过人工液体的渗透增加，30分钟后从2.5μg/cm增加到8μg/cm，180分钟后从4.4μg/cm增加到21.6μg/cm，并且与混合物的单一成分相比表现出强烈的纤溶活性。此外，这种新型混合物在时间杀灭试验中表现出对生物膜的活性以及将生长减少超过20%的能力，而在、和上，与NAC相比生长减少了超过80%。与仅使用NAC相比，flogomicina混合物还被证明能够将细菌在的非生物表面上的粘附减少超过11%。与阿莫西林联合使用时，已证明在14天后能显著提高药物的有效性，为在长期治疗中减少抗生素的每日剂量从而降低抗生素耐药性提供了一种安全且天然的方法。
