Shoji M, Kawarabayashi T, Harigaya Y, Yamaguchi H, Hirai S, Kamimura T, Sugiyama T
Department of Neurology, Gunma University School of Medicine, Japan.
Am J Pathol. 1990 Nov;137(5):1027-32.
We prepared antisera to both the N and C termini of amyloid beta-protein precursor (APP). Both antisera labeled 110 to 140 kd proteins from rat testis by immunoblotting. Northern blot analysis using oligonucleotide probes complementary to respective APPs showed that APPs expressed in rat testis contained Kunitz-type protease inhibitor domains. Immunocytochemically brain APP was localized in neurons and their processes. During sperm formation, APPs labeled by both antisera were localized only in acrosome and the growing tail of spermatids in the seminiferous tubules. This shows that APPs appear only in particular phases of spermatogenesis, that is, in the morphologic change phase from spermatid to mature sperm. Amyloid beta-protein precursors in testis may play a role in cellular differentiation or morphologic change.
我们制备了针对淀粉样β蛋白前体（APP）N端和C端的抗血清。通过免疫印迹法，两种抗血清均标记出大鼠睾丸中110至140kd的蛋白质。使用与各自APP互补的寡核苷酸探针进行Northern印迹分析表明，大鼠睾丸中表达的APP含有Kunitz型蛋白酶抑制剂结构域。免疫细胞化学显示，脑APP定位于神经元及其突起中。在精子形成过程中，两种抗血清标记的APP仅定位于生精小管中精子细胞的顶体和生长中的尾部。这表明APP仅出现在精子发生的特定阶段，即从精子细胞到成熟精子的形态变化阶段。睾丸中的淀粉样β蛋白前体可能在细胞分化或形态变化中起作用。
