人脑中三种淀粉样蛋白前体mRNA：它们在阿尔茨海默病中的差异表达。
Three types of amyloid protein precursor mRNA in human brain: their differential expression in Alzheimer's disease.
作者信息
Tanaka S, Nakamura S, Ueda K, Kameyama M, Shiojiri S, Takahashi Y, Kitaguchi N, Ito H
机构信息
Department of Neurology, Faculty of Medicine, Kyoto University, Japan.
出版信息
Biochem Biophys Res Commun. 1988 Dec 15;157(2):472-9. doi: 10.1016/s0006-291x(88)80273-0.
Three types of amyloid protein precursor (APP) mRNA, produced by alternative splicing, were detected by Northern blotting in human brains, both control and Alzheimer's disease. These mRNAs encode APP695 consisting of 695 amino acids, APP751 harboring a 56 amino acid insert homologous to a Kunitz-type trypsin inhibitor inside APP695, and APP770 containing an additional 19 amino acid insert. Another possible APP mRNA which encodes "APP714" containing a 19 amino acid insert was not found in brain samples tested. Quantitative analysis revealed that, although the relative expression levels of the three mRNAs were variable among individuals, there was no remarkable change in expression of APP695 and APP751 mRNAs in Alzheimer's disease compared with control, but that APP770 mRNA level was elevated significantly in Alzheimer's disease.
通过RNA印迹法在正常人和阿尔茨海默病患者的人脑中检测到了三种由可变剪接产生的淀粉样前体蛋白（APP）mRNA。这些mRNA分别编码由695个氨基酸组成的APP695、在APP695内部含有一个与库尼茨型胰蛋白酶抑制剂同源的56个氨基酸插入片段的APP751，以及含有另外19个氨基酸插入片段的APP770。在所检测的脑样本中未发现另一种可能编码含有19个氨基酸插入片段的“APP714”的APP mRNA。定量分析显示，尽管这三种mRNA的相对表达水平在个体间存在差异，但与正常对照相比，阿尔茨海默病患者中APP695和APP751 mRNA的表达没有明显变化，而APP770 mRNA水平在阿尔茨海默病患者中显著升高。
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