Poul F, Dunez J
Institut National de la Recherche Agronomique, Centre de Recherche de Bordeaux, Villenave d'Ornon, France.
Arch Virol. 1990;114(3-4):191-202. doi: 10.1007/BF01310748.
Thirteen monoclonal antibodies (Mabs) specific for apple chlorotic leaf spot virus (ACLSV), produced by the somatic cell hybridization technique, were used to investigate the antigenic structure of the virus. Epitope specificity studies showed that these Mabs defined in ACLSV particles seven independent antigenic domains, representing at least eight distinct epitopes. One of them was present only in virions and not in dissociated subunits. It appeared that the interaction between a Mab and the virus could, in some cases, induce conformational changes in the viral particles which enhanced the binding of others. Twenty nine virus isolates differing in geographical origin, primary hosts and symptomatology were tested with these monoclonal antibodies by ELISA. With the exception of two Mabs which did not react with three cherry isolates, and one Mab which did not react with one plum isolate, all of them recognized all ACLSV isolates tested.
通过体细胞杂交技术制备了13种针对苹果褪绿叶斑病毒（ACLSV）的单克隆抗体（Mab），用于研究该病毒的抗原结构。表位特异性研究表明，这些单克隆抗体在ACLSV颗粒中确定了7个独立的抗原结构域，代表至少8个不同的表位。其中一个仅存在于病毒粒子中，而不存在于解离的亚基中。似乎在某些情况下，单克隆抗体与病毒之间的相互作用可诱导病毒粒子的构象变化，从而增强其他抗体的结合。用这些单克隆抗体通过ELISA对29种地理来源、原始宿主和症状不同的病毒分离株进行了检测。除了两种单克隆抗体不与三种樱桃分离株反应，以及一种单克隆抗体不与一种李子分离株反应外，所有单克隆抗体都识别了所检测的所有ACLSV分离株。
