Altschuh D, Al Moudallal Z, Briand J P, Van Regenmortel M H
Mol Immunol. 1985 Mar;22(3):329-37. doi: 10.1016/0161-5890(85)90169-5.
The specificity of 18 monoclonal antibodies directed to tobacco mosaic virus (TMV) was studied by measuring their ability to bind to viral mutants, to other tobamoviruses, to dissociated viral subunits and to peptide fragments of the viral coat protein. The apparent binding specificity of the antibodies was dependent on the type of enzyme-linked immunosorbent assay used, probably because the antigens were disrupted or denatured when attached to the plastic surface of microtiter wells. The capacity of different monoclonal antibodies to detect single substitutions in the viral coat protein was used to delineate some of the topographic epitopes of TMV. By means of computer-generated images of the surface residues of the viral subunit, it was possible to identify certain clusters of residues involved in binding to some of the monoclonal antibodies. The results clearly illustrate the operational limitations encountered when monoclonal antibodies are used for elucidating the antigenic structure of proteins.
通过检测18种针对烟草花叶病毒（TMV）的单克隆抗体与病毒突变体、其他烟草花叶病毒、解离的病毒亚基以及病毒外壳蛋白肽段的结合能力，研究了它们的特异性。抗体的表观结合特异性取决于所使用的酶联免疫吸附测定类型，这可能是因为抗原附着于微量滴定孔的塑料表面时会被破坏或变性。利用不同单克隆抗体检测病毒外壳蛋白中单个取代的能力，描绘了TMV的一些拓扑表位。借助计算机生成的病毒亚基表面残基图像，能够识别与某些单克隆抗体结合有关的特定残基簇。结果清楚地说明了使用单克隆抗体阐明蛋白质抗原结构时遇到的操作限制。
