Wang Yu, Deng Tongle, Lin Lin, Pan Yuanjiang, Zheng Xiaoxiang
Department of Biomedical Engineering, Key Laboratory for Biomedical Engineering of Ministry of Education, Zhejiang University, Hangzhou 310027, PR China.
Phytother Res. 2006 Dec;20(12):1052-5. doi: 10.1002/ptr.1990.
The cytoprotective effects of various solvent extracts of Artocarpus altilis (Parkinson) Fosberg were evaluated. The cytoprotective effects were determined in human U937 cells incubated with oxidized LDL (OxLDL) using the 4-[3-(4-iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1, 3-benzene disulfonate (WST-1) assay. The results demonstrated that the ethyl acetate extract showed cytoprotective activities. To identify the main cytoprotective components, a bioassay guided isolation of the ethyl acetate extract afforded b-sitosterol (1) and six flavonoids (2-7). Their chemical structures were established on the basis of spectroscopic evidence and comparison with literature data. Of these compounds, compound 6 was obtained from A. altilis for the first time. The cytoprotective effect offers good prospects for the medicinal applications of A. altilis.
对面包树（Artocarpus altilis (Parkinson) Fosberg）各种溶剂提取物的细胞保护作用进行了评估。使用4-[3-(4-碘苯基)-2-(4-硝基苯基)-2H-5-四氮唑]-1,3-苯二磺酸盐（WST-1）检测法，在与氧化低密度脂蛋白（OxLDL）孵育的人U937细胞中测定细胞保护作用。结果表明，乙酸乙酯提取物具有细胞保护活性。为了鉴定主要的细胞保护成分，通过生物测定指导对乙酸乙酯提取物进行分离，得到了β-谷甾醇（1）和六种黄酮类化合物（2-7）。它们的化学结构是根据光谱证据并与文献数据比较确定的。在这些化合物中，化合物6首次从面包树中获得。这种细胞保护作用为面包树的药用应用提供了良好的前景。
