Barth C F, Ewert D L, Olson W C, Humphries E H
Department of Microbiology, University of Texas Southwestern Medical Center, Dallas 75235-9048.
J Virol. 1990 Dec;64(12):6054-62. doi: 10.1128/JVI.64.12.6054-6062.1990.
Infection of 1-day-old chicks with reticuloendotheliosis virus strain T induces a neoplastic disease that kills the chicks 7 to 14 days postinfection. In association with reticuloendotheliosis-associated virus (REV-A), reticuloendotheliosis virus T (REV-T) induces tumors that are predominantly immunoglobulin M (IgM) negative. We examined a variety of REV-T(REV-A)-induced tumors and tumor-derived cell lines and concluded that the principal IgM-negative tumors that develop in REV-T(REV-A)-infected chicks are neither pre-B or pre-B-pre-T but rather mature T lymphoid and myeloid. Without exception, the immunoglobulin heavy- and light-chain loci were in germ line configuration. Furthermore, the cell lines expressed neither sterile transcripts of the heavy- or light-chain immunoglobulin genes nor elevated levels of c-myb, two characteristics associated with murine pre-B lymphomas. Cell lines were also examined by using monoclonal antibodies for expression of a variety of cell surface markers expressed on B lymphocytes and/or T lymphocytes and/or myeloid cells. These reagents defined two types of IgM-negative tumor cell lines, one CIa+ CT-3+ (T lymphoid) and the other CIa+ CT-3-. By using the same approaches, tumor development was examined following REV-T(REV-A) infection at 1 and 3 weeks post-hatching of cyclophosphamide-treated chicks shown to be devoid of B-lymphoid cells. Again, the tumors that developed were either CIa+ CT-3+ (T lymphoid) or CIa+ CT-3-. Furthermore, the frequency and rate with which IgM-negative tumors developed in cyclophosphamide-treated chicks were not different from those observed in normal chicks. In 3-week-old cyclophosphamide-treated chicks, the presence of CIa+ CT-3- tumors bearing hematopoietic lineage markers, such as CLA-3 and 5M19, are most likely to have been derived from cells within the myeloid lineage.
用网状内皮增生症病毒T株感染1日龄雏鸡会引发一种肿瘤性疾病，感染后7至14天雏鸡会死亡。与网状内皮增生症相关病毒（REV-A）共同作用时，网状内皮增生症病毒T（REV-T）会诱导产生主要为免疫球蛋白M（IgM）阴性的肿瘤。我们检查了多种REV-T（REV-A）诱导的肿瘤及肿瘤衍生细胞系，得出结论：REV-T（REV-A）感染雏鸡后产生的主要IgM阴性肿瘤既不是前B细胞或前B-前T细胞，而是成熟的T淋巴细胞和髓细胞。无一例外，免疫球蛋白重链和轻链基因座呈种系构型。此外，这些细胞系既不表达重链或轻链免疫球蛋白基因的无菌转录本，也不表达升高水平的c-myb，而这两个特征与鼠前B淋巴瘤相关。还用单克隆抗体检查了细胞系，以检测B淋巴细胞和/或T淋巴细胞和/或髓细胞上表达的多种细胞表面标志物。这些试剂确定了两种IgM阴性肿瘤细胞系，一种为CIa + CT-3 +（T淋巴细胞），另一种为CIa + CT-3-。采用相同方法，在环磷酰胺处理的雏鸡孵化后1周和3周时用REV-T（REV-A）感染，检查肿瘤发生情况，这些雏鸡已被证明缺乏B淋巴细胞。同样，产生的肿瘤要么是CIa + CT-3 +（T淋巴细胞），要么是CIa + CT-3-。此外，环磷酰胺处理的雏鸡中IgM阴性肿瘤发生的频率和速率与正常雏鸡中观察到的并无差异。在3周龄经环磷酰胺处理的雏鸡中，带有造血谱系标志物（如CLA-3和5M19）的CIa + CT-3-肿瘤很可能源自髓系谱系内的细胞。
