Vennix P P, Kuijpers W, Tonnaer E L, Peters T A, Ramaekers F C
Department of Otorhinolaryngology, University of Nijmegen, The Netherlands.
Ann Otol Rhinol Laryngol. 1990 Dec;99(12):998-1004. doi: 10.1177/000348949009901213.
The expression of cytokeratins in the epithelium of the middle ear and external auditory meatus of the rat was studied on cryosections of ethylenediaminetetraacetic acid-decalcified specimens by use of a panel of monoclonal antibodies. The normal middle ear epithelium revealed a complex cytokeratin profile, including regional differences. The induction of sterile middle ear effusions resulted in increased cytokeratin expression. Infective effusions were accompanied by both quantitative and qualitative changes in the cytokeratin expression patterns. The differences observed between the cytokeratin profiles of external meatal skin and those of middle ear epithelium may form a useful tool for research into cholesteatoma development.
利用一组单克隆抗体，在乙二胺四乙酸脱钙标本的冰冻切片上研究了大鼠中耳和外耳道上皮中细胞角蛋白的表达。正常中耳上皮显示出复杂的细胞角蛋白谱，包括区域差异。无菌性中耳积液的诱导导致细胞角蛋白表达增加。感染性积液伴随着细胞角蛋白表达模式的定量和定性变化。在外耳道皮肤和中耳上皮的细胞角蛋白谱之间观察到的差异可能为胆脂瘤形成的研究提供一个有用的工具。
