在GMP - 140（血小板活化依赖颗粒外膜蛋白）介导的与活化血小板的黏附相互作用中，中性粒细胞上唾液酸的需求。

Requirement for sialic acid on neutrophils in a GMP-140 (PADGEM) mediated adhesive interaction with activated platelets.

作者信息

Corral L, Singer M S, Macher B A, Rosen S D

机构信息

Department of Anatomy, University of California, San Francisco 94143-0452.

出版信息

Biochem Biophys Res Commun. 1990 Nov 15;172(3):1349-56. doi: 10.1016/0006-291x(90)91598-m.

DOI:10.1016/0006-291x(90)91598-m

PMID:1700907

Abstract

Platelet GMP-140, along with ELAM-1 and gp90MEL, comprise the LEC-CAM family of cell-cell adhesion proteins. The three proteins demonstrate a highly related domain organization, which includes an extracellular calcium-type lectin motif. gp90MEL, a lymphocyte homing receptor, mediates lymphocyte attachment to high endothelial venules of lymph nodes through recognition of a sialylated ligand on the endothelial cells. The rosetting of neutrophils or promyelocytic HL60 cells by activated platelets is mediated by GMP-140 on the platelets. We show here that treatment of neutrophils or HL60 cells with 3 broad spectrum sialidases completely prevents rosetting. However, the Newcastle disease virus sialidase, an enzyme specific for alpha 2,3 and alpha 2,8 linkages of sialic acid does not affect rosetting of HL60 cells. These results indicate that the ligand for GMP-140 requires sialic acid and suggest that an alpha 2,6 linkage may be critical.

摘要

血小板GMP-140与ELAM-1和gp90MEL共同构成了细胞间黏附蛋白的LEC-CAM家族。这三种蛋白表现出高度相关的结构域组织，其中包括一个细胞外钙型凝集素基序。gp90MEL是一种淋巴细胞归巢受体，通过识别内皮细胞上的唾液酸化配体介导淋巴细胞与淋巴结高内皮微静脉的附着。活化血小板介导的中性粒细胞或早幼粒细胞HL60细胞的玫瑰花结形成是由血小板上的GMP-140介导的。我们在此表明，用3种广谱唾液酸酶处理中性粒细胞或HL60细胞可完全阻止玫瑰花结形成。然而，新城疫病毒唾液酸酶，一种对唾液酸的α2,3和α2,8连接具有特异性的酶，并不影响HL60细胞的玫瑰花结形成。这些结果表明，GMP-140的配体需要唾液酸，并提示α2,6连接可能至关重要。