Groves R W, Allen M H, Ross E L, Ahsan G, Barker J N, MacDonald D M
Laboratory of Applied Dermatopathology, St. John's Institute of Dermatology, UMDS, Guy's Hospital, London, United Kingdom.
Am J Pathol. 1993 Oct;143(4):1220-5.
Recent evidence suggests that interactions between endothelial selectins and tumor surface selectin ligands may be of importance in cancer metastasis. To investigate the role of such mechanisms in cutaneous tumors, whole skin biopsies were examined immunohistochemically for a variety of selectin ligands including sialyl-Lewis-X, sialyl-Lewis-A (S-Le(a)), sulfatides, and CD15. In 12 of 12 squamous cell carcinomas (SCCs), there was expression of sialyl-Lewis-X and CD15, but no tumor expressed S-Le(a). Occasional keratinocytes in eight of 12 SCCs expressed sulfatides. All selectin ligands were absent on keratinocytes in basal cell carcinomas (BCCs, n = 8) and normal skin (n = 8), with the exception of one BCC that expressed S-Le(a). E-selectin was not present in normal skin, but was strongly expressed by dermal endothelium in both SCC and BCC. Keratinocyte cell lines A431, HaCaT, and SVK14 were investigated by flow cytometry, which demonstrated sialyl-Lewis-X and S-Le(a) expression by all three, whereas normal human keratinocytes did not express these molecules. These findings suggest a potential role for selectin-mediated events in early and late metastasis, and differential expression of these ligands by BCC and SCC may explain the relatively low metastatic potential of the former.
最近的证据表明，内皮选择素与肿瘤表面选择素配体之间的相互作用在癌症转移中可能具有重要意义。为了研究此类机制在皮肤肿瘤中的作用，我们对全层皮肤活检组织进行了免疫组织化学检查，以检测包括唾液酸化路易斯-X、唾液酸化路易斯-A（S-Le(a)）、硫脂和CD15在内的多种选择素配体。在12例鳞状细胞癌（SCC）中，均有唾液酸化路易斯-X和CD15的表达，但无一例肿瘤表达S-Le(a)。12例SCC中有8例的部分角质形成细胞表达硫脂。在基底细胞癌（BCC，n = 8）和正常皮肤（n = 8）的角质形成细胞中均未检测到所有选择素配体，但有1例BCC表达S-Le(a)。E-选择素在正常皮肤中不存在，但在SCC和BCC的真皮内皮细胞中均有强烈表达。通过流式细胞术对角质形成细胞系A431、HaCaT和SVK14进行了研究，结果表明这三种细胞系均表达唾液酸化路易斯-X和S-Le(a)，而正常人角质形成细胞不表达这些分子。这些发现提示选择素介导的事件在早期和晚期转移中可能发挥潜在作用，BCC和SCC中这些配体的差异表达可能解释了前者相对较低的转移潜能。
