Sudharsan Periyasamy Thandavan, Mythili Yenjerla, Selvakumar Elangovan, Varalakshmi Palaninathan
Department of Medical Biochemistry, Dr. ALM. Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai 600113, India.
Mol Cell Biochem. 2006 Nov;292(1-2):39-44. doi: 10.1007/s11010-006-9171-1. Epub 2006 Sep 29.
Cyclophosphamide (CP), an alkylating agent widely used in cancer chemotherapy causes cardiac membrane damage. Lupeol, a pentacyclic triterpene, isolated from Crataeva nurvala stem bark and its ester, lupeol linoleate possess a wide range of medicinal properties. The effect of lupeol and its ester was evaluated in CP induced alterations in cardiac electrolytes in rats. Male albino rats of Wistar strain were categorized into 6 groups. Group I served as control. Rats in groups II, V and VI were injected intraperitoneally with a single dose of CP (200 mg/kg body weight) dissolved in saline. CP treated groups V and VI received lupeol and lupeol linoleate (50 mg/kg body weight) respectively, dissolved in olive oil for 10 days by oral gavage. At the end of the experimental period, urinary risk factors, activities of ATPases and electrolytes were measured using standard procedures. CP administered rats showed a significant decrease (P < 0.001) in the activities of ATPases. It was associated with significant alterations (P < 0.001) of electrolytes both in serum and cardiac tissue. The levels of urea, uric acid and creatinine were also significantly (P < 0.001) altered in the serum and urine. Lupeol and its ester showed reversal of the above alterations induced by CP. These findings demonstrate that the supplementation with lupeol and its ester could preserve membrane permeability, highlighting their protective effect against CP induced cardiotoxicity.
环磷酰胺（CP）是一种广泛用于癌症化疗的烷化剂，会导致心脏膜损伤。羽扇豆醇是一种从瓦氏辣木茎皮中分离出的五环三萜，其酯类羽扇豆醇亚油酸酯具有广泛的药用特性。研究评估了羽扇豆醇及其酯对CP诱导的大鼠心脏电解质变化的影响。将雄性Wistar品系白化大鼠分为6组。第一组作为对照组。第二、五和六组的大鼠腹腔注射一剂溶解于生理盐水的CP（200毫克/千克体重）。CP处理的第五和六组分别通过口服灌胃给予溶解于橄榄油中的羽扇豆醇和羽扇豆醇亚油酸酯（50毫克/千克体重），持续10天。在实验期结束时，使用标准程序测量尿液风险因素、ATP酶活性和电解质。给予CP的大鼠ATP酶活性显著降低（P < 0.001）。这与血清和心脏组织中电解质的显著变化（P < 0.001）相关。血清和尿液中的尿素、尿酸和肌酐水平也显著（P < 0.001）改变。羽扇豆醇及其酯显示出对CP诱导的上述变化的逆转作用。这些发现表明，补充羽扇豆醇及其酯可以维持膜通透性，突出了它们对CP诱导的心脏毒性的保护作用。
