羽扇豆醇通过下调 Toll 样受体 4 减轻柯萨奇病毒 B3 诱导的小鼠病毒性心肌炎。
Lupeol alleviates coxsackievirus B3-induced viral myocarditis in mice via downregulating toll-like receptor 4.
机构信息
Department of Cardiac Surgery, Wuhan Asia Heart Hospital, Wuhan, P.R. China *Ming Xu and Xiaoyong Li are co-first authors.
出版信息
J Int Med Res. 2020 Apr;48(4):300060520910908. doi: 10.1177/0300060520910908.
OBJECTIVES
To investigate the effect of lupeol in a mouse model of viral myocarditis induced by coxsackie virus B3 (CVB3).
METHODS
Mice were separated into controls (DMEM, = 20) and CVB3 infected groups (i.e., untreated CVB3 [ = 40]; CVB3 + lupeol 50 mg/kg [ = 40]; CVB3 + lupeol 100 mg/kg [ = 40]; CVB3 + small interfering RNA (siRNA)- toll-like receptor 4 (TLR4) [n = 20]; siRNA + EXP-H mice [n = 20]). Reverse transcription polymerase chain reaction (RT-PCR), western-blot assay, immunohistochemistry, enzyme-linked immunosorbent (ELISA) assay and histopathology were performed to investigate the cardioprotective role of lupeol.
RESULTS
The elevated pro-inflammatory cytokines in CVB3-infected mice (i.e., interleukin-1β [IL-1β]; interleukin-6 [IL-6]; tumour necrosis factor-α [TNF-α]) were significantly reduced by lupeol 50 or 100 mg/kg. Interestingly, the mRNA level and protein level of toll-like receptor 4 (TLR4) were inhibited by lupeol.
CONCLUSIONS
Lupeol alleviates CVB3-induced viral myocarditis and myocardial damage in mice. The underlying mechanism may due to downregulation of TLR4.
目的
研究羽扇豆醇在柯萨奇病毒 B3（CVB3）诱导的病毒性心肌炎小鼠模型中的作用。
方法
将小鼠分为对照组（DMEM，n=20）和 CVB3 感染组（即未处理的 CVB3 [n=40]；CVB3+羽扇豆醇 50mg/kg [n=40]；CVB3+羽扇豆醇 100mg/kg [n=40]；CVB3+小干扰 RNA（siRNA）- Toll 样受体 4（TLR4）[n=20]；siRNA+EXP-H 小鼠 [n=20]）。采用逆转录聚合酶链反应（RT-PCR）、Western blot 分析、免疫组织化学、酶联免疫吸附（ELISA）测定和组织病理学方法研究羽扇豆醇的心脏保护作用。
结果
羽扇豆醇可显著降低 CVB3 感染小鼠升高的促炎细胞因子（即白细胞介素-1β[IL-1β]；白细胞介素-6[IL-6]；肿瘤坏死因子-α[TNF-α]）。有趣的是，羽扇豆醇可抑制 TLR4 的 mRNA 水平和蛋白水平。
结论
羽扇豆醇可减轻 CVB3 诱导的病毒性心肌炎和小鼠心肌损伤。其潜在机制可能与 TLR4 的下调有关。
Zotero 插件