Matovelo J A, Landsverk T, Sund R B
Department of Pathology, Norwegian College of Veterinary Medicine, Oslo.
APMIS. 1990 Oct;98(10):887-95. doi: 10.1111/j.1699-0463.1990.tb05011.x.
Structural features associated with reversibility of lesions induced by deoxycholic acid (DOC) were studied by electron microscopy and immunofluorescence techniques. Tied jejunal loops were incubated in vivo with 2.5-20 mmol/l DOC in isotonic solution. Immediately after this treatment, or after a recovery period of 15 or 150 minutes following washout of the bile acid, the loops were excised and processed. DOC produced epithelial lesions whose severity and reversibility were concentration-dependent. Ultrastructural features associated with the reversibility of the lesions were particularly apparent in specimens exposed to 10-20 mmol/l DOC. These features included cell flattening with the formation of thin, veil-like structures into the eroded area by cells at the edges of the erosions. Immunofluorescence studies showed a redistribution of actin and cytokeratin filaments to the margins and leading edges of the flattened cells. It is suggested that cell flattening and migration are responsible for the rapid morphological recovery of the injured epithelium. Actin and cytokeratin appear to be instrumental in the remodelling and migration.
采用电子显微镜和免疫荧光技术研究了与脱氧胆酸（DOC）诱导损伤的可逆性相关的结构特征。将结扎的空肠袢在体内与等渗溶液中2.5 - 20 mmol/L的DOC一起孵育。在该处理后立即，或在冲洗掉胆汁酸后15或150分钟的恢复期后，切除肠袢并进行处理。DOC产生上皮损伤，其严重程度和可逆性呈浓度依赖性。与损伤可逆性相关的超微结构特征在暴露于10 - 20 mmol/L DOC的标本中尤为明显。这些特征包括细胞扁平化，侵蚀边缘的细胞形成薄的、面纱样结构进入侵蚀区域。免疫荧光研究显示肌动蛋白和细胞角蛋白丝重新分布到扁平化细胞的边缘和前缘。提示细胞扁平化和迁移是受损上皮快速形态恢复的原因。肌动蛋白和细胞角蛋白似乎在重塑和迁移中起作用。
