Matovelo J A, Sund R B, Landsverk T
Department of Pathology, Norwegian College of Veterinary Medicine, Oslo.
APMIS. 1989 Sep;97(9):798-810. doi: 10.1111/j.1699-0463.1989.tb00481.x.
Whereas many studies deal with teh deleterious effects of unconjugated deoxycholic acid on epithelial morphology, few are concerned with the reversibility of these effects, the subject of the present study. Tied jejunal loops in the rat were incubated for 30 minutes with deoxycholic acid (2.5-20 mmol/litre) in isotonic solution. Immediately after this treatment, or after a subsequent recovery period of 15 or 150 minutes following wash out of the bile acid, the loops were excised, fixed and examined by light microscopy and scanning electron microscopy. Deoxycholic acid produced epithelial lesions whose severity and reversibility depended on the concentration applied. However, even the severely affected epithelium obtained by treatment at 10-20 mmol/litre was reverted to normal within 150 minutes, and a substantial normalisation was observed already after 15 minutes. An exception to this rapid restoration of epithelial morphology and integrity was noted in villi which had suffered necrosis of lamina propria. The revertion of epithelial pathology was paralleled with a normalisation of glucose absorption, of the potassium ion and protein content of the loop fluid, and of the paracellular epithelial permeability as measured with 3-H-poly-ethylenglycol. Morphometry showed that deoxycholic acid caused villous atrophy without affectin the crypt length. The extent and reversibility of this atrophy depended on dose and recovery time as above. It is suggested that the remarkably fast morphological restitution proceeds mainly by process involving cellular remodelling and migration.
尽管许多研究探讨了未结合的脱氧胆酸对上皮形态的有害影响，但很少有研究关注这些影响的可逆性，而这正是本研究的主题。将大鼠的空肠袢在等渗溶液中与脱氧胆酸（2.5 - 20 mmol/升）孵育30分钟。在该处理后立即，或在冲洗掉胆汁酸后的15或150分钟的后续恢复期后，切除肠袢，固定并通过光学显微镜和扫描电子显微镜检查。脱氧胆酸产生上皮病变，其严重程度和可逆性取决于所用的浓度。然而，即使是通过10 - 20 mmol/升处理获得的严重受影响的上皮，在150分钟内也恢复正常，并且在15分钟后就已观察到明显的正常化。在固有层发生坏死的绒毛中，注意到上皮形态和完整性的这种快速恢复存在例外情况。上皮病理学的恢复与葡萄糖吸收、肠袢液中钾离子和蛋白质含量以及用³H - 聚乙二醇测量的细胞旁上皮通透性的正常化平行。形态计量学表明，脱氧胆酸导致绒毛萎缩而不影响隐窝长度。这种萎缩的程度和可逆性如上述取决于剂量和恢复时间。有人提出，这种非常快速的形态恢复主要通过涉及细胞重塑和迁移的过程进行。
