Feirabend H K
Department of Physiology, University of Leiden, The Netherlands.
Eur J Morphol. 1990;28(2-4):169-223.
The cytoarchitecture of the cerebellum has been studied in chicken embryos from day 3-20 using serial sections stained with cresylviolet, haematoxylin-eosin and toluidine blue. Three periods have been distinguished in cerebellar development on a basis of cytoarchitectonic characteristics. Of these periods the middle one, which lasts from the 8th to the 15th day, is marked by two subsequent transient longitudinal cytoarchitectonic patterns in the cortical anlage. The first pattern, which exists between days 8 and 11, consists of 4 longitudinal Purkinje cell clusters (of the first order) at either side of the midline. The second pattern, which is most distinct and complete during embryonic days 12-14, is caused by specific localizations of otherwise few, early inwardly migrating granule cells from the external cerebellar matrix (so-called granule raphes), which pass through the layer of Purkinje cell clusters of the first order and thus subdivide these latter into smaller entities: Purkinje cell clusters of the second order. The number of these latter (6 or 7 and 11 or 12 in the anterior and posterior lobes, respectively) correspond to the number of parasagittal modules, which can be discerned on a basis of the organization of fiber connections of the adult cerebellar cortex. Thanks to this similarity various hypotheses can be formulated concerning the significance of the transient cytoarchitectonic patterns in the primitive cortex for the genesis of the modular organization of the cerebellum.
利用用甲酚紫、苏木精-伊红和甲苯胺蓝染色的连续切片，对3至20日龄鸡胚的小脑细胞结构进行了研究。根据细胞结构特征，在小脑发育过程中区分出三个阶段。在这些阶段中，中间阶段从第8天持续到第15天，其特征是在皮质原基中出现两种相继的短暂纵向细胞结构模式。第一种模式存在于第8天至第11天之间，由中线两侧的4个纵向浦肯野细胞簇（一级）组成。第二种模式在胚胎第12至14天最为明显和完整，是由少数早期从外部小脑基质向内迁移的颗粒细胞（所谓的颗粒缝）的特定定位引起的，这些颗粒细胞穿过一级浦肯野细胞簇层，从而将后者细分为更小的实体：二级浦肯野细胞簇。后者的数量（前叶和后叶分别为6或7个和11或12个）与矢状旁模块的数量相对应，矢状旁模块可根据成年小脑皮质纤维连接的组织情况辨别出来。由于这种相似性，可以就原始皮质中短暂的细胞结构模式对小脑模块化组织形成的意义提出各种假设。
