Roeling T A, Feirabend H K
Laboratory of Anatomy and Embryology, University of Leiden, The Netherlands.
Glia. 1988;1(6):398-402. doi: 10.1002/glia.440010607.
The possible relation between glial fibers and the formation of longitudinal granule cell migration patterns that occur in the cerebellar anlage of the chicken was investigated by immunocytochemistry of vimentin (monoclonal antibody) and glial fibrillary acidic protein (polyclonal antibody against GFAP, PGF) on fixed and unfixed brain tissues. In addition, neuronal development was studied with a monoclonal antibody for neurofilament. Vimentin was present in radial and tangential fibers in the cerebellar anlage during granule cell migration in almost all parts of the anlage. However, no specific topographic relation of vimentin and GFAP to the migration pattern of granule cells was observed. In adults, Bergmann fibers and astroglia were stained with vimentin antiserum and not with GFAP antiserum. Conclusions are that radial fibers do not determine the formation of longitudinal cytoarchitectonic patterns in the chick cerebellum and that vimentin is the main cytoskeletal component of Bergmann fibers and astroglial cells in embryonic and adult chicken cerebellum.
通过对固定和未固定脑组织进行波形蛋白（单克隆抗体）和胶质纤维酸性蛋白（抗GFAP多克隆抗体，PGF）的免疫细胞化学研究，探讨了胶质纤维与鸡小脑原基中纵向颗粒细胞迁移模式形成之间的可能关系。此外，用抗神经丝单克隆抗体研究了神经元发育。在颗粒细胞迁移过程中，波形蛋白存在于小脑原基几乎所有部位的径向和切向纤维中。然而，未观察到波形蛋白和GFAP与颗粒细胞迁移模式有特定的拓扑关系。在成年鸡中，Bergmann纤维和星形胶质细胞用波形蛋白抗血清染色，而不用GFAP抗血清染色。结论是，径向纤维并不决定鸡小脑纵向细胞构筑模式的形成，波形蛋白是胚胎期和成年期鸡小脑Bergmann纤维和星形胶质细胞的主要细胞骨架成分。
