转录因子COUP-TF1对乙肝病毒增强子II的基因型依赖性激活或抑制
Genotype-dependent activation or repression of HBV enhancer II by transcription factor COUP-TF1.
作者信息
Fischer Silke F, Schmidt Katja, Fiedler Nicola, Glebe Dieter, Schüttler Christian, Sun Jianguang, Gerlich Wolfram H, Repp Reinald, Schaefer Stephan
机构信息
Institut für Medizinische Virologie, Justus-Liebig-Universität Giessen, Germany.
出版信息
World J Gastroenterol. 2006 Oct 7;12(37):6054-8. doi: 10.3748/wjg.v12.i37.6054.
AIM
To study the expression of HBV enhancer II by transcription factor COUP-TF1.
METHODS
In order to study the regulation of HBV variants in the vicinity of the NRRE we cloned luciferase constructs containing the HBV enhancer II from variants and from HBV genotypes A and D and cotransfected them together with expression vectors for COUP-TF1 into HepG2 cells.
RESULTS
Our findings show that enhancer II of HBV genotype A is also repressed by COUP-TF1. In contrast, two different enhancer II constructs of HBV genotype D were activated by COUP-TF1. The activation was independent of the NRRE because a natural variant with a deletion of nt 1763-1770 was still activated by COUP-TF1.
CONCLUSION
Regulation of transcription of the HBV genome seems to differ among HBV genomes derived from different genotypes. These differences in transcriptional control among HBV genotypes may be the molecular basis for differences in the clinical course among HBV genotypes.
目的
研究转录因子COUP-TF1对乙肝病毒增强子II的表达情况。
方法
为研究NRRE附近乙肝病毒变异体的调控，我们从变异体以及乙肝病毒A基因型和D基因型中克隆了包含乙肝病毒增强子II的荧光素酶构建体，并将它们与COUP-TF1的表达载体一起共转染到HepG2细胞中。
结果
我们的研究结果表明，乙肝病毒A基因型的增强子II也受到COUP-TF1的抑制。相比之下，乙肝病毒D基因型的两种不同增强子II构建体被COUP-TF1激活。这种激活与NRRE无关，因为一个缺失nt 1763 - 1770的天然变异体仍被COUP-TF1激活。
结论
不同基因型乙肝病毒基因组的转录调控似乎存在差异。乙肝病毒基因型之间转录控制的这些差异可能是不同基因型乙肝病毒临床病程差异的分子基础。
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