Davidson M, Nasisse M, Kornegay J
Department of Companion Animal and Special Species Medicine, College of Veterinary Medicine, North Carolina State University, Raleigh.
J Comp Pathol. 1990 Aug;103(2):125-34. doi: 10.1016/s0021-9975(08)80169-7.
Intermediate filament expression of various cell types in the adult canine normal and gliotic retina was determined by an immunoperoxidase method of using monoclonal antibodies on aldehyde-fixed tissues. In the normal retina, vimentin was present in astrocytes in the nerve fibre layer, horizontal cell processes, and Müller cell fibres from the internal limiting membrane to the outer nuclear layer. Neurofilamentous axons were noted in the nerve fibre, inner plexiform layer, and outer plexiform layer, although the degree of staining intensity varied among the three molecular weight neurofilament antisera used. Glial fibrillary acidic protein (GFAP) staining was confined to the nerve fibre and ganglion cell layer; this was interpreted as representing fibrous astrocytes. Astrocyte density varied according to retinal topography with an increased number around retinal blood vessels and in the peripapillary retina. Quantitative, but not qualitative differences in staining for vimentin and the neurofilaments were noted in degenerative, gliotic retinas. In common with several other mammalian species previously studied, the canine Müller cells accumulate or express GFAP under pathological conditions involving a gliotic response.
采用免疫过氧化物酶法，使用单克隆抗体对醛固定组织进行检测，以确定成年犬正常和胶质化视网膜中各种细胞类型的中间丝表达情况。在正常视网膜中，波形蛋白存在于神经纤维层的星形胶质细胞、水平细胞突起以及从内界膜到外核层的 Müller 细胞纤维中。在神经纤维层、内网状层和外网状层中可观察到神经丝轴突，不过在所使用的三种分子量神经丝抗血清中，染色强度有所不同。胶质纤维酸性蛋白（GFAP）染色局限于神经纤维层和神经节细胞层；这被解释为代表纤维性星形胶质细胞。星形胶质细胞密度根据视网膜地形图而有所变化，在视网膜血管周围和视乳头周围视网膜中数量增加。在退行性、胶质化视网膜中，波形蛋白和神经丝的染色存在定量差异，但无定性差异。与之前研究的其他几种哺乳动物一样，犬的 Müller 细胞在涉及胶质化反应的病理条件下会积累或表达 GFAP。
