Nuttall W O, Ladds P W, Daniels P W
Graduate School of Tropical Veterinary Science, James Cook University of North Queensland, Townsville, Australia.
J Comp Pathol. 1990 Aug;103(2):221-8. doi: 10.1016/s0021-9975(08)80178-8.
The peroxidase-antiperoxidase method was used to quantify immunoglobulin (Ig)-containing cells at the host-tumour interface in ovine aural squamous cell carcinoma lesions at various stages of development. IgM-containing cells were never observed; only scattered, but strongly staining, IgA-containing cells were present. IgG-containing cells predominated; their prevalence increased from 0.17 +/- 0.34 cells per high power field (hpf; mean +/- standard deviation) in precursor lesions to a maximum of 13.6 +/- 2.6 cells per hpf in overt tumours which had not metastasized. Their prevalence in overt tumours which had metastasized was 4.6 +/- 3.9 cells per hpf. Quantitation of pyroninophils in sections stained by the methyl green-pyronin method showed a greater prevalence of plasma cells than IgG-containing cells, but a similar pattern of change was apparent. Mast cells were most prevalent in early stages, but no significant change in eosinophil numbers was observed.
采用过氧化物酶-抗过氧化物酶法对处于不同发育阶段的绵羊耳鳞状细胞癌病变的宿主-肿瘤界面含免疫球蛋白（Ig）的细胞进行定量分析。从未观察到含IgM的细胞；仅存在散在但染色强烈的含IgA细胞。含IgG的细胞占主导；其发生率从前体病变中的每高倍视野（hpf；平均值±标准差）0.17±0.34个细胞增加到未转移的明显肿瘤中的每hpf最多13.6±2.6个细胞。在已转移的明显肿瘤中其发生率为每hpf 4.6±3.9个细胞。用甲基绿-派洛宁法染色的切片中嗜派洛宁细胞的定量分析显示，浆细胞的发生率高于含IgG的细胞，但变化模式相似。肥大细胞在早期最为普遍，但未观察到嗜酸性粒细胞数量有显著变化。
