Pate M, Svara T, Gombac M, Paller T, Zolnir-Dovc M, Emersic I, Prodinger W M, Bartos M, Zdovc I, Krt B, Pavlik I, Cvetnić Z, Pogacnik M, Ocepek M
Institute of Microbiology and Parasitology, Vetinary Faculty, Gerbiceva, Ljubljana, Slovenia.
J Vet Med B Infect Dis Vet Public Health. 2006 Oct;53(8):387-92. doi: 10.1111/j.1439-0450.2006.01000.x.
In the autumn of 2004, tuberculosis caused by Mycobacterium caprae occurred in a zoo in Slovenia. A dromedary camel (Camelus dromedarius) was killed after a history of progressive emaciation. Necropsy findings indicated disseminated tuberculosis, which was confirmed by cultivation of M. caprae. Consequently, a tuberculin skin test was performed in all epidemiologically linked animals and another dromedary camel and six bison (Bison bison) were positive and killed. Mycobacterium caprae was isolated from two bison while M. scrofulaceum and Mycobacterium spp. were found in two other bison, respectively. The second dromedary camel was found to be negative for mycobacteria under both microscopic and culture tests. The isolates were investigated with commercial identification kits, IS6110 PCR, IS6110 restriction fragment length polymorphism analysis, spoligotyping and mycobacterial interspersed repetitive units typing. Genotyping results revealed that the dromedary camel and the two bison were infected by the same M. caprae.
2004年秋，斯洛文尼亚一家动物园出现了由山羊分枝杆菌引起的结核病。一头单峰骆驼（ Camelus dromedarius ）在经历了渐进性消瘦后死亡。尸检结果显示为播散性结核病，通过培养山羊分枝杆菌得以确诊。因此，对所有存在流行病学关联的动物进行了结核菌素皮肤试验，另一头单峰骆驼和六头美洲野牛（ Bison bison ）呈阳性并被扑杀。从两头美洲野牛中分离出了山羊分枝杆菌，另外两头美洲野牛分别检出了瘰疬分枝杆菌和分枝杆菌属。经显微镜检查和培养试验，发现第二头单峰骆驼的分枝杆菌呈阴性。使用商业鉴定试剂盒、IS6110聚合酶链反应、IS6110限制性片段长度多态性分析、间隔寡核苷酸分型和分枝杆菌散布重复单位分型对分离株进行了研究。基因分型结果显示，单峰骆驼和两头美洲野牛感染的是同一株山羊分枝杆菌。
