Runge M, Binder A, Gurr E, Fischer M, Meier J, Kirchhoff H
Institut für Mikrobiologie und Tierseuchen, Tierärztliche Hochschule, Hannover.
Zentralbl Bakteriol. 1990 Sep;273(4):531-8. doi: 10.1016/s0934-8840(11)80460-3.
Polyclonal and monoclonal antibodies against Mycoplasma (M.) arthritidis membranes were investigated in the indirect immunofluorescence test (IIFT) and enzyme immunoassay (EIA) for their reactivity with rat and human chondrocytes as well as with rat skin fibroblasts. The monoclonal antibody A 79 gave positive reactions with rat chondrocytes in the IIFT up to a dilution off 1: 128 and in EIA up to a dilution of 1:16. In the EIA, the monoclonal antibodies A 31, A 32 and A 58 recognized M. arthritidis as well as rat and human chondrocyte membrane antigens up to dilutions of 1:128 and 1:256 and rat skin fibroblasts up to dilutions of 1:32/64. In the IIFT, the whole surfaces of the rat and human chondrocytes were strongly fluorescing after the treatment with the polyclonal antiserum against M. arthritidis. The monoclonal antibody A 79 caused weak fluorescence (rat chondrocytes) or no fluorescence (human chondrocytes) on the surface of the chondrocytes but a stronger fluorescence on areas around and between them. From these results it can be concluded that A 79 probably reacts with antigens of the chondrocyte matrix.
研究了抗关节炎支原体（M. arthritidis）膜的多克隆抗体和单克隆抗体在间接免疫荧光试验（IIFT）和酶免疫测定（EIA）中与大鼠和人类软骨细胞以及大鼠皮肤成纤维细胞的反应性。单克隆抗体A 79在IIFT中与大鼠软骨细胞的阳性反应可达1:128稀释度，在EIA中可达1:16稀释度。在EIA中，单克隆抗体A 31、A 32和A 58识别关节炎支原体以及大鼠和人类软骨细胞膜抗原的稀释度可达1:128和1:256，识别大鼠皮肤成纤维细胞的稀释度可达1:32/64。在IIFT中，用抗关节炎支原体的多克隆抗血清处理后，大鼠和人类软骨细胞的整个表面强烈荧光。单克隆抗体A 79在软骨细胞表面引起弱荧光（大鼠软骨细胞）或无荧光（人类软骨细胞），但在它们周围和之间的区域有较强荧光。从这些结果可以得出结论，A 79可能与软骨细胞基质的抗原发生反应。
