Pinho Ricardo A, Andrades Michael E, Oliveira Marcos R, Pirola Aline C, Zago Morgana S, Silveira Paulo C L, Dal-Pizzol Felipe, Moreira José Cláudio F
Departamento de Bioquímica, Centro de Estudos em Estresse Oxidativo, UFRGS, Porto Alegre, RS, Brazil.
Cell Biol Int. 2006 Oct;30(10):848-53. doi: 10.1016/j.cellbi.2006.03.011. Epub 2006 Aug 24.
The association between physical exercise and oxidative damage in the skeletal musculature has been the focus of many studies in literature, but the balance between superoxide dismutase and catalase activities and its relation to oxidative damage is not well established. Thus, the aim of the present study was to investigate the association between regular treadmill physical exercise, oxidative damage and antioxidant defenses in skeletal muscle of rats. Fifteen male Wistar rats (8-12 months) were randomly separated into two groups (trained n=9 and untrained n=6). Trained rats were treadmill-trained for 12 weeks in progressive exercise (velocity, time, and inclination). Training program consisted in a progressive exercise (10 m/min without inclination for 10 min/day). After 1 week the speed, time and inclination were gradually increased until 17 m/min at 10% for 50 min/day. After the training period animals were killed, and gastrocnemius and quadriceps were surgically removed to the determination of biochemical parameters. Lipid peroxidation, protein oxidative damage, catalase, superoxide dismutase and citrate synthase activities, and muscular glycogen content were measured in the isolated muscles. We demonstrated that there is a different modulation of CAT and SOD in skeletal muscle in trained rats when compared to untrained rats (increased SOD/CAT ratio). TBARS levels were significantly decreased and, in contrast, a significant increase in protein carbonylation was observed. These results suggest a non-described adaptation of skeletal muscle against exercise-induced oxidative stress.
体育锻炼与骨骼肌氧化损伤之间的关联一直是文献中众多研究的焦点，但超氧化物歧化酶和过氧化氢酶活性之间的平衡及其与氧化损伤的关系尚未明确确立。因此，本研究的目的是调查大鼠骨骼肌中定期跑步机体育锻炼、氧化损伤与抗氧化防御之间的关联。15只雄性Wistar大鼠（8 - 12个月）被随机分为两组（训练组n = 9，未训练组n = 6）。训练组大鼠在递增运动（速度、时间和坡度）下进行12周的跑步机训练。训练计划包括递增运动（10米/分钟，无坡度，每天10分钟）。1周后，速度、时间和坡度逐渐增加，直至达到17米/分钟，坡度为10%，每天50分钟。训练期结束后处死动物，通过手术取出腓肠肌和股四头肌以测定生化参数。在分离的肌肉中测量脂质过氧化、蛋白质氧化损伤、过氧化氢酶、超氧化物歧化酶和柠檬酸合酶活性以及肌肉糖原含量。我们证明，与未训练的大鼠相比，训练大鼠骨骼肌中CAT和SOD存在不同的调节（SOD/CAT比值增加）。TBARS水平显著降低，相反，观察到蛋白质羰基化显著增加。这些结果表明骨骼肌对运动诱导的氧化应激存在一种未被描述的适应性变化。
