Campaner Adriana Bittencourt, Piato Sebastião, Galvão Maria Antonieta L, dos Santos Roberto Euzébio, Nadais Ricardo F
Department of Obstetrics and Gynecology, Medical Science College of Santa Casa of São Paulo, Brazil.
J Low Genit Tract Dis. 2006 Oct;10(4):223-8. doi: 10.1097/01.lgt.0000225891.03613.f7.
Compare the numerical densities of intraepithelial Langerhans cells of uterine cervix of women affected by cervical intraepithelial neoplasia grade 3 (CIN 3) with their smoking habits.
A total of 71 conization specimens of women affected by CIN 3 were separated in 3 groups according to their smoking habits (smokers, nonsmokers, and former smokers). The identification of the Langerhans cells was performed by immunohistochemical analysis using antibodies to S100 protein. The number of intraepithelial Langerhans cells was counted at x400 magnification under a light microscope, and a 10-field count was performed in areas of CIN 3 of each section. Results were expressed as number of cells per square millimeter of epithelium.
There was no significant difference in the number of Langerhans cells per square millimeter of epithelium in areas affected by CIN 3 among the 3 groups (p = .5). There was also no significant difference in the number of cigarettes smoked per day (p = .09), duration of consumption (p = .34), total amount of cigarettes smoked during the whole life (p = .18), and duration of abstention (p = .2).
It was not shown that smoking reduces the number of intraepithelial Langerhans cells in the cervix of women affected by CIN 3.
比较宫颈上皮内瘤变3级（CIN 3）女性患者宫颈上皮内朗格汉斯细胞的数量密度与其吸烟习惯之间的关系。
将71例CIN 3女性患者的锥形切除标本根据吸烟习惯分为3组（吸烟者、非吸烟者和既往吸烟者）。使用抗S100蛋白抗体通过免疫组织化学分析鉴定朗格汉斯细胞。在光学显微镜下以400倍放大倍数计数上皮内朗格汉斯细胞的数量，对每个切片的CIN 3区域进行10个视野的计数。结果以每平方毫米上皮细胞数表示。
3组中受CIN 3影响区域每平方毫米上皮内朗格汉斯细胞的数量无显著差异（p = 0.5）。每日吸烟量（p = 0.09）、吸烟持续时间（p = 0.34）、终生吸烟总量（p = 0.18）和戒烟持续时间（p = 0.2）也无显著差异。
未显示吸烟会减少CIN 3女性患者宫颈上皮内朗格汉斯细胞的数量。
