Department of Obstetrics and Gynecology, Medical Science College of Santa Casa of São Paulo, Av. Rebouças, 1511 ap. 142, Jd. América, São Paulo, SP 05401-200, Brazil.
Int J Gynecol Pathol. 2009 Nov;28(6):549-53. doi: 10.1097/PGP.0b013e3181aa232d.
The objective of our study was to evaluate the effect of smoking on intraepithelial Langerhans cells and T and B lymphocytes in normal cervical epithelium. A total of 47 women who underwent hysterectomies because of uterine leiomyomata were investigated. Thirty-two individuals were nonsmokers and 15 were current smokers. A segment of tissue was taken from the cervix after the removal of the uterus and was formalin-fixed and paraffin-embedded for histologic analysis. The identification of the Langerhans cells and T and B lymphocytes was carried out by immunohistochemical analysis. The number of intraepithelial Langerhans cells was expressed by the amount of cells per square millimeter of epithelium. For T and B lymphocytes, a score expressed the cell count. The comparison of the number of intraepithelial Langerhans cells between smoking and nonsmoking women showed a significant difference (P=0.045), but it did not occur in relation to the number of T and B cells between the 2 groups. There was also no significant difference in relation to the number of cigarettes smoked per day, time of consumption, and total amount of cigarettes smoked throughout the lifetime. It was shown that smoking reduces the number of intraepithelial Langerhans cells in a normal cervix but does not influence the number of T and B lymphocytes.
本研究旨在评估吸烟对正常宫颈上皮内朗格汉斯细胞及 T、B 淋巴细胞的影响。共纳入因子宫肌瘤行子宫切除术的 47 名女性患者。其中 32 名非吸烟者，15 名吸烟者。子宫切除术后取宫颈组织一段，福尔马林固定、石蜡包埋用于组织学分析。应用免疫组化方法鉴定朗格汉斯细胞及 T、B 淋巴细胞。上皮内朗格汉斯细胞的数量以每平方毫米上皮细胞内的细胞数表示。T、B 淋巴细胞的数量以细胞计数的评分表示。吸烟与非吸烟女性的上皮内朗格汉斯细胞数量比较差异有统计学意义（P=0.045），但两组间 T、B 细胞数量无差异。每日吸烟支数、吸烟时间、吸烟总量与上皮内朗格汉斯细胞数量亦无相关性。提示吸烟可减少正常宫颈上皮内朗格汉斯细胞数量，但对 T、B 淋巴细胞数量无影响。
