Cook R M
Beecham Pharmaceuticals Research Division, Biosciences Research Centre, Epsom, Surrey, U.K.
Clin Exp Allergy. 1990 Sep;20(5):511-7. doi: 10.1111/j.1365-2222.1990.tb03143.x.
Intravenous injection of Sephadex G200 particles into rats on days 0, 2 and 5 caused an increase in eosinophil numbers in blood and lung tissues. Peak numbers were obtained on days 3-12 and thereafter declined to approach control values by day 21. The rise in eosinophil numbers was paralleled by an increase in lung cell fragility as measured by a transient reduction in the number of viable cells isolated from parenchymatous tissue following the digestion of lung fragments in vitro. This decrease in lung cell viability was not seen in rats given a single injection of Sephadex. Dexamethasone, dapsone and isoprenaline given before each injection of Sephadex reduced lung and blood eosinophil numbers and prevented lung cell death. Aspirin and indomethacin were without effect. Incubation of normal lung tissues with disrupted peritoneal eosinophils reduced the numbers of viable cells recovered. No such effects were seen using intact eosinophils and disrupted or intact neutrophils and mononuclear cells. This system provides a model of lung cell damage associated with eosinophil infiltration in vivo.
在第0、2和5天给大鼠静脉注射葡聚糖G200颗粒，会导致血液和肺组织中嗜酸性粒细胞数量增加。在第3至12天达到峰值，此后到第21天下降至接近对照值。嗜酸性粒细胞数量的增加与肺细胞脆性增加平行，肺细胞脆性通过体外消化肺碎片后从实质组织中分离出的活细胞数量短暂减少来衡量。在单次注射葡聚糖的大鼠中未观察到肺细胞活力的这种下降。在每次注射葡聚糖之前给予地塞米松、氨苯砜和异丙肾上腺素可降低肺和血液中嗜酸性粒细胞数量，并防止肺细胞死亡。阿司匹林和吲哚美辛无效。用破裂的腹腔嗜酸性粒细胞孵育正常肺组织会减少回收的活细胞数量。使用完整的嗜酸性粒细胞以及破裂或完整的中性粒细胞和单核细胞未观察到此类效果。该系统提供了一种与体内嗜酸性粒细胞浸润相关的肺细胞损伤模型。
