Cook R M, Musgrove N R, Smith H
Beecham Pharmaceuticals, Epsom, Surrey, UK.
Pulm Pharmacol. 1989;2(4):185-90. doi: 10.1016/0952-0600(89)90019-7.
Treatment of rats with a single intravenous injection of 0.5 mg Sephadex G200 caused a blood and lung eosinophilia first detected on day 2, peaking on day 7 and declining to control levels by days 28 and 84 respectively. The increase in eosinophil numbers was accompanied by the development of pulmonary granulomas reaching their maximum size 2-7 days after Sephadex treatment and persisting for 84 days. This reaction was characterised by an early infiltration with polymorphonuclear leucocytes followed by increased numbers of mononuclear cells. Few eosinophils were associated with the Sephadex particles but large numbers were found in the adventitial layers of the lung. There was an increase in the size of the granulomas in rats given Sephadex on days 0, 2 and 5 compared with the response in rats given a single injection of Sephadex. The inflammation was more intense and more eosinophils were detected in the blood, alveolar tissues and adventitia. The effects of drugs given before each injection of Sephadex were evaluated. Dexamethasone, dapsone and isoprenaline reduced the blood and tissue eosinophilia but had no effect on the number of granulomas. Dexamethasone and dapsone, but not isoprenaline caused a slight reduction in the size of the inflammatory reaction around the Sephadex particles. Indomethacin had no effect on the granuloma formation, nor did it reduce the eosinophilia. Isoprenaline and indomethacin increased the proportion of eosinophils associated with the granuloma, although in neither instance did this reach significance.
给大鼠单次静脉注射0.5毫克葡聚糖G200后，在第2天首次检测到血液和肺部嗜酸性粒细胞增多，第7天达到峰值，到第28天和第84天分别降至对照水平。嗜酸性粒细胞数量的增加伴随着肺部肉芽肿的形成，在葡聚糖处理后2 - 7天达到最大尺寸，并持续84天。这种反应的特征是早期有嗜中性粒细胞浸润，随后单核细胞数量增加。与葡聚糖颗粒相关的嗜酸性粒细胞很少，但在肺的外膜层发现大量嗜酸性粒细胞。与单次注射葡聚糖的大鼠相比，在第0、2和5天给予葡聚糖的大鼠肉芽肿尺寸增大。炎症更强烈，在血液、肺泡组织和外膜中检测到更多嗜酸性粒细胞。评估了每次注射葡聚糖前给予药物的效果。地塞米松、氨苯砜和异丙肾上腺素可降低血液和组织中的嗜酸性粒细胞增多，但对肉芽肿数量没有影响。地塞米松和氨苯砜，但不是异丙肾上腺素，使葡聚糖颗粒周围的炎症反应尺寸略有减小。吲哚美辛对肉芽肿形成没有影响，也没有降低嗜酸性粒细胞增多。异丙肾上腺素和吲哚美辛增加了与肉芽肿相关的嗜酸性粒细胞比例，尽管在这两种情况下都没有达到显著水平。
