Izumi Naohiro, Nagaoka Taiji, Mori Fumihiko, Sato Eiichi, Takahashi Atsushi, Yoshida Akitoshi
Department of Ophthalmology, Asahikawa Medical College, Asahikawa, Japan.
Departments of Ophthalmology and Surgery, Scott & White Eye Institute, Texas A&M University Health Science Center, Temple, TX, USA.
Jpn J Ophthalmol. 2006 Sep-Oct;50(5):465-468. doi: 10.1007/s10384-006-0344-y.
Nitric oxide (NO) plays an important role in homeostatic vasodilation and the regulation of blood flow. On the other hand, excess release of NO causes various vascular complications. There are only a few reports on the relationship between plasma NO levels and microvascular complications, especially diabetic retinopathy (DR) in patients with type 2 diabetes. The purpose of this study was to determine the relationship between plasma NO levels and DR.
In a prospective study, blood samples were obtained from 36 patients with diabetes and no diabetic retinopathy (NDR), 43 patients with nonproliferative diabetic retinopathy (NPDR), 18 patients with proliferative diabetic retinopathy (PDR), and 40 subjects without diabetes mellitus, who served as controls. The levels of plasma NOx (nitrite and nitrate), the stable metabolites of NO, were measured by high-performance liquid chromatography with the Griess method.
The plasma NOx levels were 92.8 +/- 16.0, 70.2 +/- 6.8, 90.3 +/- 9.1, and 53.8 +/- 6.1 micromol/l in patients with NDR, NPDR, or PDR, and in the controls, respectively. The plasma NOx levels in the three diabetic groups were significantly higher than those in the control group (P < 0.05 in each case).
The increased plasma NO levels in patients with type 2 diabetes indicate that NO may be associated with the pathogenesis of DR.
一氧化氮（NO）在稳态血管舒张和血流调节中起重要作用。另一方面，NO的过度释放会导致各种血管并发症。关于血浆NO水平与微血管并发症之间的关系，尤其是2型糖尿病患者的糖尿病视网膜病变（DR），只有少数报道。本研究的目的是确定血浆NO水平与DR之间的关系。
在一项前瞻性研究中，从36例无糖尿病视网膜病变（NDR）的糖尿病患者、43例非增殖性糖尿病视网膜病变（NPDR）患者、18例增殖性糖尿病视网膜病变（PDR）患者以及40例无糖尿病的受试者（作为对照）中采集血样。采用高效液相色谱法结合格里斯方法测定血浆NOx（亚硝酸盐和硝酸盐）水平，NOx是NO的稳定代谢产物。
NDR、NPDR或PDR患者以及对照组的血浆NOx水平分别为92.8±16.0、70.2±6.8、90.3±9.1和53.8±6.1 μmol/L。三个糖尿病组的血浆NOx水平均显著高于对照组（每组P<0.05）。
2型糖尿病患者血浆NO水平升高表明NO可能与DR的发病机制有关。
