Jiang Qi, Lyu Xue-Man, Yuan Yi, Wang Ling
Department of Nephrology, China-Japan Union Hospital of Jilin University, Changchun 130033, P.R. China.
Department of Ophthalmology, China-Japan Union Hospital of Jilin University, Changchun 130033, P.R. China.
Biosci Rep. 2017 Mar 27;37(2). doi: 10.1042/BSR20160589. Print 2017 Apr 28.
To investigate the roles of plasma in the pathogenic process of Type 2 diabetes (T2D) with diabetic retinopathy (DR). T2D patients included patients without DR (NDR) group, patients with non-proliferative/background DR (BDR) group and patients with proliferative DR (PDR) group. Healthy individuals served as control group. Fasting plasma glucose (FPG), glycosylated haemoglobin (HbA1c), triacylglycerol (TG), total cholesterol (TC), urine creatinine (Cr), fasting blood glucose (FBG), blood urea nitrogen (BUN), low-density lipoprotein cholesterol (LDL-C), fasting insulin (FINS) and plasma expression were measured. Quantitative real-time PCR (qRT-PCR) was applied to detect expression. Pearson analysis was used to conduct correlation analysis and receiver operating characteristic (ROC) curve was used to analyse the diagnostic value of in T2D with DR. Compared with the control group, FBG and HbA1c increased in the NDR group; compared with the control and NDR groups, disease course, HbA1c, FPG levels and homoeostasis model assessment of insulin resistance (HOMA-IR) were increased in the BDR and PDR groups; and compared with the BDR group, disease course, HbA1c and FPG levels were higher in the PDR group. expression was higher in the BDR group than the control group, and higher in the PDR group than the BDR group. expression was positively related with disease course, HbA1C, FPG and HOMA-IR, and had diagnostic value for T2D with DR and PDR. The plasma expression was increased in the development of T2D with DR and can be used as an indicator for the severity of T2D with DR.
探讨血浆在2型糖尿病（T2D）合并糖尿病视网膜病变（DR）发病过程中的作用。T2D患者包括无DR患者（NDR组）、非增殖性/背景性DR患者（BDR组）和增殖性DR患者（PDR组）。健康个体作为对照组。检测空腹血糖（FPG）、糖化血红蛋白（HbA1c）、三酰甘油（TG）、总胆固醇（TC）、尿肌酐（Cr）、空腹血糖（FBG）、血尿素氮（BUN）、低密度脂蛋白胆固醇（LDL-C）、空腹胰岛素（FINS）及血浆表达水平。采用定量实时聚合酶链反应（qRT-PCR）检测表达。采用Pearson分析进行相关性分析，采用受试者工作特征（ROC）曲线分析在T2D合并DR中的诊断价值。与对照组相比，NDR组的FBG和HbA1c升高；与对照组和NDR组相比，BDR组和PDR组的病程、HbA1c、FPG水平及胰岛素抵抗稳态模型评估（HOMA-IR）升高；与BDR组相比，PDR组的病程、HbA1c和FPG水平更高。BDR组的表达高于对照组，PDR组的表达高于BDR组。表达与病程、HbA1C、FPG和HOMA-IR呈正相关，对T2D合并DR和PDR具有诊断价值。血浆表达在T2D合并DR的发生发展过程中升高，可作为T2D合并DR严重程度的指标。
