Huang Ling, Manandhar Pradeep, Byun Kyung-Eun, Chase P Bryant, Hong Seunghun
Department of Physics and MARTECH, Florida State University, Tallahassee, Florida 32306, USA.
Langmuir. 2006 Oct 10;22(21):8635-8. doi: 10.1021/la061008a.
We report a new strategy to selectively assemble and align filamentous actin (F-actin) onto desired locations on a solid substrate with a specific structural polarity. In this strategy, biotinylated gelsolin caps the structural minus end of F-actin so that the F-actin binds onto a streptavidin pattern with a specific structural polarity. We also demonstrate that an electric field can be utilized to align bound F-actin along a desired direction. This can be one of the major technical breakthroughs toward the assembly of nanomechanical systems based on myosin biomotors.
