Manandhar Pradeep, Huang Ling, Grubich Justin R, Hutchinson John W, Chase P Bryant, Hong Seunghun
Langmuir. 2005 Apr 12;21(8):3213-6. doi: 10.1021/la047227i.
The capability of assembling biomotors onto specific locations of solid substrates is a key for development of biomotor-based nanomechanical systems. We developed a method to direct the assembly of the heavy meromyosin fragment from rabbit skeletal muscle myosin onto specific locations of Au substrates utilizing surface molecular patterns. In this strategy, chemically directed patterns of streptavidin were achieved to direct highly specific assembly of biotinylated heavy meromyosin on the substrates--a strategy applicable for patterning a variety of biotinylated molecules--while BSA was utilized to avoid nonspecific adsorption. In vitro motility assays of filament sliding were used to confirm functionality of assembled actomyosin.
将生物马达组装到固体基质的特定位置的能力是基于生物马达的纳米机械系统发展的关键。我们开发了一种方法，利用表面分子图案将兔骨骼肌肌球蛋白的重酶解肌球蛋白片段定向组装到金基质的特定位置。在该策略中，实现了链霉亲和素的化学定向图案，以指导生物素化重酶解肌球蛋白在基质上的高度特异性组装——这是一种适用于多种生物素化分子图案化的策略——同时利用牛血清白蛋白避免非特异性吸附。使用细丝滑动的体外运动分析来确认组装的肌动球蛋白的功能。
