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通过杂交蛋白分析对大肠杆菌青霉素结合蛋白1B（PBP 1B）单克隆抗体的构象表位进行定位：对PBP 1B三级结构的影响

Mapping of conformational epitopes of monoclonal antibodies against Escherichia coli penicillin-binding protein 1B (PBP 1B) by means of hybrid protein analysis: implications for the tertiary structure of PBP 1B.

作者信息

Den Blaauwen T, Pas E, Edelman A, Spratt B G, Nanninga N

机构信息

Department of Molecular Cell Biology, University of Amsterdam, The Netherlands.

出版信息

J Bacteriol. 1990 Dec;172(12):7284-8. doi: 10.1128/jb.172.12.7284-7288.1990.

DOI:10.1128/jb.172.12.7284-7288.1990
PMID:1701431
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC210859/
Abstract

We have analyzed the location of the epitope areas of the four monoclonal antibody groups against penicillin-binding protein 1B (PBP 1B; T. den Blaauwen, F. B. Wientjes, A. H. J. Kolk, B. G. Spratt, and N. Nanninga, J. Bacteriol. 171:1393-1401). They could be specified by studying monoclonal antibody binding patterns to amino- and carboxy-terminal truncated PBP 1B molecules. Monoclonal antibodies against conformational epitopes, with the exception of one epitope area, did not recognize PBP 1B molecules that had not been translocated across the membrane. Apparently, translocation is required for PBP 1B to fully obtain its native conformation.

摘要

我们分析了针对青霉素结合蛋白1B（PBP 1B；T. den Blaauwen、F. B. Wientjes、A. H. J. Kolk、B. G. Spratt和N. Nanninga，《细菌学杂志》171:1393 - 1401）的四组单克隆抗体的表位区域位置。通过研究单克隆抗体与氨基末端和羧基末端截短的PBP 1B分子的结合模式，可以确定这些表位区域。除了一个表位区域外，针对构象表位的单克隆抗体不能识别未跨膜转运的PBP 1B分子。显然，PBP 1B要完全获得其天然构象需要转运。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3022/210859/e0b6db4bbb8b/jbacter00166-0689-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3022/210859/ab18f3585d8a/jbacter00166-0688-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3022/210859/28eb9afabe3d/jbacter00166-0688-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3022/210859/99c5791aca7d/jbacter00166-0688-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3022/210859/e0b6db4bbb8b/jbacter00166-0689-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3022/210859/ab18f3585d8a/jbacter00166-0688-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3022/210859/28eb9afabe3d/jbacter00166-0688-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3022/210859/99c5791aca7d/jbacter00166-0688-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3022/210859/e0b6db4bbb8b/jbacter00166-0689-a.jpg

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Mapping of conformational epitopes of monoclonal antibodies against Escherichia coli penicillin-binding protein 1B (PBP 1B) by means of hybrid protein analysis: implications for the tertiary structure of PBP 1B.通过杂交蛋白分析对大肠杆菌青霉素结合蛋白1B（PBP 1B）单克隆抗体的构象表位进行定位：对PBP 1B三级结构的影响
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