Pietramaggiori Giorgio, Kaipainen Arja, Czeczuga Joshua M, Wagner Christopher T, Orgill Dennis P
Division of Plastic Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA.
Wound Repair Regen. 2006 Sep-Oct;14(5):573-80. doi: 10.1111/j.1743-6109.2006.00164.x.
Fresh platelet concentrates are used in many centers to treat recalcitrant wounds. To extend the therapeutic shelf-life of platelets, we analyzed the wound-healing effects of fresh-frozen and freeze-dried (FD) platelet-rich plasma (PRP) using a diabetic mouse model. Db/db mice with 1.0 cm2 dorsal excisional wounds (n = 15/group) were treated with a single application of FD PRP (1.2 x 10(6) platelets/microL) with or without a stabilization solution, and compared with wounds treated with fresh-frozen, sonicated PRP, and untreated wounds. Granulation tissue area, thickness, and wound size were analyzed 9 days posttreatment. Immunostained sections were quantified for vascularity and proliferation using antiplatelet endothelial cell adhesion molecule I and antiproliferating cell nuclear antigen antibodies. The results showed that all PRP preparations increased granulation tissue formation as assessed by surface coverage, thickness, and angiogenic response, when compared with untreated wounds. In addition, wounds treated with FD PRP, and biochemically stabilized FD PRP, exhibited higher proliferative levels. The possibility to deliver growth factors using platelets, and the potential to extend the shelf-life of platelet concentrates makes freeze-drying methods particularly suitable for enhanced wound care.
许多医疗中心使用新鲜血小板浓缩物来治疗顽固性伤口。为了延长血小板的治疗保质期，我们使用糖尿病小鼠模型分析了新鲜冷冻和冻干（FD）富血小板血浆（PRP）的伤口愈合效果。对背部有1.0平方厘米切除伤口的db/db小鼠（每组n = 15）单次应用FD PRP（1.2×10⁶个血小板/微升），添加或不添加稳定溶液，并与用新鲜冷冻、超声处理的PRP处理的伤口以及未处理的伤口进行比较。在治疗后9天分析肉芽组织面积、厚度和伤口大小。使用抗血小板内皮细胞粘附分子I和抗增殖细胞核抗原抗体对免疫染色切片的血管生成和增殖进行定量分析。结果表明，与未处理的伤口相比，所有PRP制剂均通过表面覆盖、厚度和血管生成反应评估增加了肉芽组织形成。此外，用FD PRP和生化稳定的FD PRP处理的伤口表现出更高的增殖水平。利用血小板递送生长因子的可能性以及延长血小板浓缩物保质期的潜力使得冻干方法特别适用于增强伤口护理。
