Dawod Ahmed, Miro Jordi, Elbaz Hamed T, Fahmy Hossam, Abdoon Ahmed S
Husbandry and Animal Wealth Development Department, Faculty of Veterinary Medicine, University of Sadat City, Menofia 32897, Egypt.
Department of Animal Medicine and Surgery, Veterinary Faculty, Autonomous University of Barcelona, E-08193 Barcelona, Spain.
Animals (Basel). 2021 Apr 14;11(4):1123. doi: 10.3390/ani11041123.
This study was designed to examine the effect of the intrauterine infusion of platelet-rich plasma (PRP) or equine lyophilized growth factor (L-GF) on the follicular growth, endometrial thickness, estrus cycle length, and pregnancy rate in purebred Arabian mares. A total of 73 purebred Arabian mares who experienced repeat breeding for three successive cycles were randomly divided into the following three groups: control group, without treatment; second Group (PRP group), in which mares were intrauterine infused with 20 mL of fresh PRP on the second day after the end of physic estrus phase; and the third group (L-GF Group), consisting of mares that were intrauterine infused with 20 mL of reconstituted lyophilized horse platelets growth factors (L-GF) on the second day after the estrus phase. The results showed no significant difference between control and treated groups in the diameter of the preovulatory follicles during the post treatment cycle. The endometrium thickness increased significantly in the L-GF and PRP groups ahead of the non-treated group. Intrauterine L-GF or PRP administration shortened the estrus cycle length. A higher pregnancy rate was recorded in the L-GF and PRP treated mares. In conclusion, the intrauterine infusion of L-GF or PRP increased the endometrial thickness and pregnancy rate and could be used to improve fertility in Arabian purebred mares who experienced from repeat breeding.
本研究旨在探讨子宫内输注富血小板血浆（PRP）或马冻干生长因子（L-GF）对纯种阿拉伯母马卵泡生长、子宫内膜厚度、发情周期长度和妊娠率的影响。总共73匹连续三个周期反复配种的纯种阿拉伯母马被随机分为以下三组：对照组，不进行治疗；第二组（PRP组），在生理发情期结束后的第二天，给母马子宫内输注20 mL新鲜PRP；第三组（L-GF组），由在发情期后的第二天子宫内输注20 mL复溶冻干马血小板生长因子（L-GF）的母马组成。结果显示，在治疗后的周期中，对照组和治疗组之间排卵前卵泡直径无显著差异。L-GF组和PRP组的子宫内膜厚度比未治疗组显著增加。子宫内给予L-GF或PRP缩短了发情周期长度。L-GF和PRP治疗的母马妊娠率更高。总之，子宫内输注L-GF或PRP增加了子宫内膜厚度和妊娠率，可用于提高反复配种的阿拉伯纯种母马的繁殖力。
