Turkmani Aun, Ioannidis Alexandros, Christidou Athanasia, Psaroulaki Anna, Loukaides Feidias, Tselentis Yiannis
Department of Clinical Bacteriology, Parasitology, Zoonoses, and Geographical Medicine, Faculty of Medicine, University of Crete, Greece.
Ann Clin Microbiol Antimicrob. 2006 Oct 2;5:24. doi: 10.1186/1476-0711-5-24.
Brucellosis is an endemic disease present in many countries worldwide, but it is rare in Europe and North America. Nevertheless brucella is included in the bacteria potentially used for bioterrorism. The aim of this study was the investigation of the antibiotic susceptibility profile of brucella isolates from areas of the eastern Mediterranean where it has been endemic.
The susceptibilities of 74 Brucella melitensis isolates derived from clinical samples (57) and animal products (17) were tested in vitro. The strains originate from Crete (59), Cyprus (10), and Syria (5). MICs of tetracycline, rifampicin, streptomycin, gentamicin, norfloxacin, ciprofloxacin, levofloxacin, trimethoprim/sulfamethoxazole, ampicillin, amoxicillin/clavulanic acid, and erythromycin were detected by E-test method. The NCCLS criteria for slow growing bacteria were considered to interpret the results.
All the isolates were susceptible to tetracycline, streptomycin, gentamicin, ciprofloxacin, norfloxacin, and levofloxacin. Two isolates presented reduced susceptibility to rifampicin (MIC value: 1.5 mg/l) and eight to SXT (MIC values: 0.75-1.5 mg/l). Erythromycin had the highest (4 mg/l) MIC90value and both norfloxacin and erythromycin the highest (1.5 mg/l) MIC50 value.
Brucella isolates remain susceptible in vitro to most antibiotics used for treatment of brucellosis. The establishment of a standardized antibiotic susceptibility method for Brucella spp would be useful for resistance determination in these bacteria and possible evaluation of bioterorism risks.
布鲁氏菌病是一种在世界许多国家流行的疾病，但在欧洲和北美较为罕见。然而，布鲁氏菌被列入可能用于生物恐怖主义的细菌名单。本研究的目的是调查来自地中海东部流行地区的布鲁氏菌分离株的抗生素敏感性谱。
对74株从临床样本（57株）和动物产品（17株）中分离出的羊种布鲁氏菌进行体外药敏试验。这些菌株分别来自克里特岛（59株）、塞浦路斯（10株）和叙利亚（5株）。采用E-test法检测四环素、利福平、链霉素、庆大霉素、诺氟沙星、环丙沙星、左氧氟沙星、甲氧苄啶/磺胺甲恶唑、氨苄西林、阿莫西林/克拉维酸和红霉素的最低抑菌浓度（MIC）。结果判断采用美国国家临床实验室标准化委员会（NCCLS）针对生长缓慢细菌的标准。
所有分离株对四环素、链霉素、庆大霉素、环丙沙星、诺氟沙星和左氧氟沙星均敏感。2株分离株对利福平敏感性降低（MIC值：1.5mg/l），8株对甲氧苄啶/磺胺甲恶唑敏感性降低（MIC值：0.75 - 1.5mg/l）。红霉素的MIC90值最高（4mg/l），诺氟沙星和红霉素的MIC50值最高（1.5mg/l）。
布鲁氏菌分离株在体外对大多数用于治疗布鲁氏菌病的抗生素仍敏感。建立布鲁氏菌属标准化抗生素敏感性检测方法，对于确定这些细菌的耐药性以及评估生物恐怖主义风险可能具有重要意义。
