中国内蒙古乌兰察布市人源布鲁氏菌羊种菌分离株的体外抗菌药敏试验
In vitro antimicrobial susceptibility testing of human Brucella melitensis isolates from Ulanqab of Inner Mongolia, China.
作者信息
Liu Zhi-Guo, Di Dong-Dong, Wang Miao, Liu Ri-Hong, Zhao Hong-Yan, Piao Dong-Ri, Zhao Zhong-Zhi, Hao Yong-Qing, Du Ya-Nan, Jiang Hai, Cui Bu-Yun, Xia Xian-Zhu
机构信息
College of Veterinary Medical Inner Mongolia Agriculture University, Hohhot, 010018, China.
Ulanqab Centre for Endemic Disease Prevention and Control, Health and Family Planning Commission of Ulanqab, Ulanqab west Road, Jining, 012000 Inner, Mongolia.
出版信息
BMC Infect Dis. 2018 Jan 16;18(1):43. doi: 10.1186/s12879-018-2947-6.
BACKGROUND
Brucellosis is an endemic disease in the Inner Mongolia Autonomous Region of China and Ulanqab exhibits the highest prevalence of brucellosis in this region. Due to the complex nature of Brucellosis, a cure for this disease has proven to be elusive. Furthermore, the reduced susceptibility of Brucella spp. to antimicrobial agents has been reported as a potential cause of therapeutic failure. However, detailed in vitro antimicrobial susceptibility patterns pertaining to Brucella isolates from this region have not yet been published. The aim of this study was to evaluate the antibiotic susceptibility profile of Brucella melitensis clinical isolates from Ulanqab, Inner Mongolia, China.
METHODS
A total of 85 B. melitesis isolates were obtained from humans in Ulanqab of Inner Mongolia, China; the antimicrobial susceptibility of 85 clinical isolates to nine antibiotics was assessed using the E-test method according to the CLSI (Clinical and Laboratory Standards Institute) guidelines.
RESULTS
All of the tested isolates were susceptible to minocycline, sparfloxacin, doxycycline, tetracycline, ciprofloxacin, gentamicin and levofloxacin. Resistance to rifampin and cotrimoxazole was observed in 1.0% (1/85) and 7.0% (6/85) of the isolates, respectively. However, rpoB gene mutations were not observed in single isolates exhibiting resistance to rifampin.
CONCLUSIONS
We observed that B. melitensis isolates are susceptible to the majority of the tested antibiotics. Furthermore, minocycline and sparfloxacin exhibited extremely high bactericidal effects in relation to the B. melitensis isolates. The sensitivity of commonly used drugs for the treatment of brucellosis should be regularly monitored. To the best of our knowledge, this is the first report of rifampin and cotrimoxazole resistant isolates of B. melitensis in China. In summary, based on the findings from this study, we suggest that antibiotic administration and use should be rationalized to prevent future drug resistance.
背景
布鲁氏菌病是中国内蒙古自治区的一种地方病，乌兰察布市是该地区布鲁氏菌病患病率最高的地区。由于布鲁氏菌病的复杂性，已证实难以治愈该病。此外，有报道称布鲁氏菌属对抗菌药物的敏感性降低是治疗失败的潜在原因。然而，关于该地区布鲁氏菌分离株的详细体外抗菌药敏模式尚未发表。本研究的目的是评估来自中国内蒙古乌兰察布市的羊种布鲁氏菌临床分离株的抗生素敏感性谱。
方法
从中国内蒙古乌兰察布市的人类中总共获得了85株羊种布鲁氏菌分离株；根据临床和实验室标准协会（CLSI）指南，使用E-test法评估了85株临床分离株对9种抗生素的抗菌敏感性。
结果
所有测试分离株均对米诺环素、司帕沙星、多西环素、四环素、环丙沙星、庆大霉素和左氧氟沙星敏感。分别在1.0%（1/85）和7.0%（6/85）的分离株中观察到对利福平和复方新诺明的耐药性。然而，在对利福平耐药的单个分离株中未观察到rpoB基因突变。
结论
我们观察到羊种布鲁氏菌分离株对大多数测试抗生素敏感。此外，米诺环素和司帕沙星对羊种布鲁氏菌分离株表现出极高的杀菌效果。应定期监测治疗布鲁氏菌病常用药物的敏感性。据我们所知，这是中国首次报道羊种布鲁氏菌对利福平和复方新诺明耐药的分离株。总之，基于本研究的结果，我们建议应合理使用抗生素以防止未来出现耐药性。
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