Alamian Saeed, Dadar Maryam, Etemadi Afshar, Afshar Davoud, Alamian Mohammad Mehdi
Department of Brucellosis, Razi Vaccine and Serum Research Institute (RVSRI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.
Department of Microbiology and Virology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
Iran J Microbiol. 2019 Oct;11(5):363-367.
Brucellosis is a widespread zoonotic disease with a high prevalence in both animals and humans. The present study was aimed to evaluate the susceptibility of strains isolated from human clinical specimens against commonly used antimicrobial agents.
A total of 360 blood specimens were collected during 2016-2018 and subjected to culture and spp. identification. The classical biotyping for isolates was performed according to Alton and coworker's guidelines. Antimicrobials susceptibility test carried out using disk diffusion and minimal inhibitory concentration (MIC) methods.
In this study, sixty strains were isolated from blood samples (16%) and all them belonged to biovar 1. Majority of the tested antibacterial agents, excepting ampicillin-sulbactam had an effective activity against isolates in E-test (MIC) and disk diffusion method. Moreover, probable resistance to rifampin and ampicillin-sulbactam were observed in 60 (100%), 1 (1.7%), 11 (18.4%) and 2 (3.4%) isolates, respectively.
Our data suggest that the efficacy of commonly used antibiotics for brucellosis treatment should be regularly monitored. In conclusion, appropriate precaution should be exercised in the context of antibiotic administration to prevent future antibiotic resistance.
布鲁氏菌病是一种广泛传播的人畜共患病，在动物和人类中均有较高的患病率。本研究旨在评估从人类临床标本中分离出的菌株对常用抗菌药物的敏感性。
在2016 - 2018年期间共收集了360份血液标本，并进行培养和菌种鉴定。根据奥尔顿及其同事的指南对分离株进行经典生物分型。采用纸片扩散法和最低抑菌浓度（MIC）法进行抗菌药物敏感性试验。
在本研究中，从血液样本中分离出60株菌株（16%），所有菌株均属于生物变种1。除氨苄西林 - 舒巴坦外，大多数受试抗菌药物在E试验（MIC）和纸片扩散法中对分离株具有有效活性。此外，分别在60株（100%）、1株（1.7%）、11株（18.4%）和2株（3.4%）分离株中观察到对利福平和氨苄西林 - 舒巴坦的可能耐药性。
我们的数据表明，应定期监测常用抗生素治疗布鲁氏菌病的疗效。总之，在使用抗生素时应采取适当的预防措施，以防止未来出现抗生素耐药性。
