Langedijk J P, Schalken J J, Tersmette M, Huisman J G, Meloen R H
Central Veterinary Institute, Lelystad, The Netherlands.
J Gen Virol. 1990 Nov;71 ( Pt 11):2609-14. doi: 10.1099/0022-1317-71-11-2609.
Antibody-binding sites were mapped on all overlapping nonapeptides of the major core protein p24 of human immunodeficiency virus type 1 (HIV-1) using murine monoclonal antibodies (MAbs) and sheep and rabbit polyclonal antibodies raised against HIV-1/H9 (strain IIIB) viral lysate and antibodies obtained from humans infected with HIV-1. The binding sites were mapped to various distinct regions of this protein. After superimposition of the antibody-binding sites on a proposed model of p24 of HIV-1, these sites appeared to be located on the surface of the protein on loops, turns and coils of p24 but, unexpectedly, not on the major part of the predicted 'puff'. Little reaction was found with the inaccessible anti-parallel beta-barrel. These results are the first experimental evidence for the validity of the structure proposed for p24 of HIV-1.
利用鼠单克隆抗体（MAbs）、针对HIV-1/H9（IIIB株）病毒裂解物产生的羊和兔多克隆抗体以及从感染HIV-1的人类获得的抗体，将抗体结合位点定位在人类免疫缺陷病毒1型（HIV-1）主要核心蛋白p24的所有重叠九肽上。这些结合位点被定位到该蛋白的不同区域。在将抗体结合位点叠加到HIV-1 p24的一个提议模型上后，这些位点似乎位于p24的环、转角和卷曲结构的蛋白质表面，但出乎意料的是，不在预测的“凸起”的主要部分。与难以接近的反平行β桶几乎没有反应。这些结果是HIV-1 p24所提议结构有效性的首个实验证据。
