Curtis Adam, Aitchison Gregor, Tsapikouni Theodora
Centre for Cell Engineering, University of Glasgow, Glasgow G12 8QQ, UK.
J R Soc Interface. 2006 Dec 22;3(11):753-6. doi: 10.1098/rsif.2006.0132.
Though actin filaments running across the cell (transverse actin) have been occasionally reported for epithelial cells in groups and for cells growing on fibres, there has been no report heretofore of transverse actin in cells grown on planar substrata. This paper describes evidence in support of this possibility derived from actin staining, polarization microscopy and force measurements. The paper introduces two new methods for detecting the orientation and activity of contractile elements in cells. The orthogonal actin is most obvious in cells grown on groove ridge structures, but can be detected in cells grown on flat surfaces.
尽管曾有报道偶尔提及，在成群的上皮细胞以及在纤维上生长的细胞中存在贯穿细胞的肌动蛋白丝（横向肌动蛋白），但迄今为止，尚未有关于在平面基质上生长的细胞中存在横向肌动蛋白的报道。本文描述了通过肌动蛋白染色、偏振显微镜观察和力测量所获得的支持这一可能性的证据。本文还介绍了两种检测细胞中收缩元件方向和活性的新方法。正交肌动蛋白在生长于沟脊结构上的细胞中最为明显，但在生长于平面表面的细胞中也能检测到。
